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Bov.hsc70.RSET.a1-554.wt(NarI)

Plasmid

#12533

Depositor

David McKay

Article

Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

Insert

Bovine 70 kDa heat shock cognate protein fragment amino acids 1-554

Use

Tags

Expression

Bacterial

Mutation

deleted carboxy-terminal residues 555-650 of bovi…

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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MAPRE1 F9.3 gRNA

Plasmid

#90746

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPRE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAPRE1 (Guide Designation F9.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pPKm-163

Plasmid

#104854

Purpose

pcDNA3 - pCMV - PIF3 - GBD, expressing PIF3 and GBD, under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PIF3-GBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf86

Plasmid

#12774

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf86

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV tdTomato R4-R3

Plasmid

#62151

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

tdTomato

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV Luc2 (FF-Luciferase) R4-R3

Plasmid

#62169

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Luciferase

Use

Luciferase; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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PBK D1.3 gRNA

Plasmid

#90824

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PBK (Guide Designation D1.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-SMO-1(GG)

Plasmid

#87054

Purpose

Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

smo-1 (smo-1 Nematode)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The last aa was removed to render processed SMO-1…

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV eGFP L1-R5

Plasmid

#62142

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
DCT.VV.Orf84

Plasmid

#12772

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf84

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV iRFP L5-L2

Plasmid

#62157

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pM-ErbB4CTF-PY3 mutant

Plasmid

#17799

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292, …

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pECE M2-SNX17 S437A

Plasmid

#69812

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf144

Plasmid

#12832

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf144

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pENTR4-RBPMS

Plasmid

#59387

Purpose

Entry vector with RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf174

Plasmid

#12862

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf174

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR SV40 PuroR L3-L2

Plasmid

#62164

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Puromycin

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
r-22. pT7-7-SNCA-9Asn

Plasmid

#107297

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-9Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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t2. pT7-7-SNCA-5Lys

Plasmid

#107294

Purpose

Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Lys (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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r-6. pT7-7-SNCA-2Asn

Plasmid

#107296

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-2Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge