Addgene

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pDUAL-Pnmt1-Ost4-GFP-MTalpha

Plasmid

#154370

Purpose

Yeast expression of Ost4

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Ost4 (ost4 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-P41nmt1-Sad1-GFP-MTn

Plasmid

#154367

Purpose

Yeast expression of Sad1

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Sad1 (sad1 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (G112P)

Plasmid

#135496

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

G112P

Promoter

CMV

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (L156P)

Plasmid

#135497

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

L156P

Promoter

CMV

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-SFXN2

Plasmid

#132256

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SFXN2 (SFXN2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCEP4-myc-H2-Dd (Y84C/C121S/A139C)

Plasmid

#136063

Purpose

Mammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Y84C/C121S/A139C

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B15-VN

Plasmid

#135514

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*15:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*15:01 (HLA-B Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B57-VN

Plasmid

#135515

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*57:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*57:1 (HLA-B Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd-VN

Plasmid

#135517

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Dd

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

C121S

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-TM-VC

Plasmid

#135499

Purpose

Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

TAPBPR (TAPBPL Human)

Use

Tags

Luminal/Extracellular FLAG epitope tag, Signal pe…

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B8-VN

Plasmid

#135511

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-B*08:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*08:01 (HLA-B Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (I52P)

Plasmid

#135486

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (I52P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

I52P

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (Y59P)

Plasmid

#135492

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y59P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y59P

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (D61P)

Plasmid

#135493

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (D61P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

D61P

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (V67P)

Plasmid

#135494

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (V67P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

V67P

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (Y84P)

Plasmid

#135495

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y84P

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (E166P)

Plasmid

#135498

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

E166P

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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p21-P369S-MEFV

Plasmid

#134712

Purpose

Lentiviral expression of human MEFV P369S after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mutation

p.P369S

Promoter

TRE2 (Dox-inducible)

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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p21-M680I-MEFV

Plasmid

#134711

Purpose

Lentiviral expression of human MEFV M680I after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mutation

p.M680I

Promoter

TRE2 (Dox-inducible)

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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p21-M694I-MEFV

Plasmid

#134710

Purpose

Lentiviral expression of human MEFV M694I after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mutation

p.M694I

Promoter

TRE2 (Dox-inducible)

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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