Skip to main content
Addgene
Showing: 10881 - 10900 of 20487 results

  1. pLKO_HSV.S.V5_mCherry-NLS

    Plasmid
    #178278
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.S.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178278-plasmid-map-sequence-id-353142

    Enlarge

  2. pLKO_HSV.Ollas.V5_mCherry-NLS

    Plasmid
    #178277
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.Ollas.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178277-plasmid-map-sequence-id-353139

    Enlarge

  3. pLKO_HSV.NWS.S_mCherry-NLS

    Plasmid
    #178273
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.NWS.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178273-plasmid-map-sequence-id-353133

    Enlarge

  4. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113687
    Purpose
    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113687-plasmid-map-sequence-id-231825

    Enlarge

  5. PB-PE SpG

    Plasmid
    #179081
    Purpose
    All-in-one prime editor piggyBac transposon, SpG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179081-plasmid-map-sequence-id-352652

    Enlarge

  6. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid
    #113692
    Purpose
    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113692-plasmid-map-sequence-id-229557

    Enlarge

  10. pLKO_AU1.HA.VSVg_mCherry-NLS

    Plasmid
    #178220
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178220-plasmid-map-sequence-id-352764

    Enlarge

  11. pLKO_AU1.HA.S_mCherry-NLS

    Plasmid
    #178219
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178219-plasmid-map-sequence-id-352763

    Enlarge

  12. pLKO_AU1.HA.HSV_mCherry-NLS

    Plasmid
    #178216
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.HSV and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178216-plasmid-map-sequence-id-352760

    Enlarge

  13. pLKO_AU1.C.VSVg_mCherry-NLS

    Plasmid
    #178214
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178214-plasmid-map-sequence-id-352758

    Enlarge

  14. pLKO_AU1.C.S_mCherry-NLS

    Plasmid
    #178213
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178213-plasmid-map-sequence-id-352757

    Enlarge

  15. pLKO_AU1.C.HSV_mCherry-NLS

    Plasmid
    #178210
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.HSV and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178210-plasmid-map-sequence-id-352753

    Enlarge

  16. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113700
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113700-plasmid-map-sequence-id-231558

    Enlarge

  20. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Expression
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

    Enlarge

Showing: 10881 - 10900 of 20487 results