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pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2

Plasmid

#62130

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

CRISPR; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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SNX31-PX (1-106)

Plasmid

#119110

Purpose

Bacterial expression of human phox homology (PX) domain, SNX31-PX (1-106)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX31-PX (1-106) (SNX31 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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SNX27-PX (156-265)

Plasmid

#119106

Purpose

Bacterial expression of human phox homology (PX) domain, SNX27-PX (156-265)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX27-PX (156-265) (SNX27 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 iRFP L3-L2

Plasmid

#62160

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_mup53_Ex5

Plasmid

#85535

Purpose

Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Nanog-t2A-mCherry

Plasmid

#127541

Purpose

Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Nanog (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Codes for the last 110 a.a of Nanog

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pQTEV-EPSTI1

Plasmid

#34826

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

epithelial stromal interaction 1 (EPSTI1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-FLJ23445

Plasmid

#15429

Depositor

Ronald Conaway, Joan Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

FLJ23445 (Thoc7 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 FLAG mTOR 1968-2549 S2035T

Plasmid

#23000

Depositor

Jing Huang

Article

Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 7. ()

Insert

mTOR (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S2035T aa 1968-2549 only

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV iRFP L1-R5

Plasmid

#62156

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pRS316-VTC4

Plasmid

#108691

Purpose

VTC4 sequence expressed in yeast plasmid

Depositor

Michael Downey

Article

Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

Insert

Vacuolar Transporter Chaperone 4

Use

Tags

Expression

Yeast

Mutation

Promoter

VTC4 (native promoter)

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 n.s shRNA L1-R5

Plasmid

#62135

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

non-silencing (scrambled) shRNA

Use

RNAi; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET23a_mod_RBPMS2_FL

Plasmid

#59394

Purpose

Plasmid for bacterial overexpression of RBPMS2

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Use

Tags

6xHis, FLAG, HA, and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV eGFP R4-R3

Plasmid

#62141

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg2 (CG510)

Plasmid

#139456

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf35

Plasmid

#12723

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf35 (J3R Vaccinia Virus)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-Barkor (∂CC)

Plasmid

#24404

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of amino acids 91-180 of the coiled-coil…

Promoter

Available since

May 20, 2010

Availability

Academic Institutions and Nonprofits only
pet28a_RBPMS_1_129

Plasmid

#65738

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-129

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_100

Plasmid

#65735

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-100

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pVETL-Cmcs-Flag-Ataxin3Q80-C14A

Plasmid

#22121

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C14A

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only