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Plasmid#100091PurposedCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expressionDepositorTypeEmpty backboneUseCRISPRTags3XFlag-NLS-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only
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pET-pAG-Hia5
Plasmid#174373PurposePlasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein AG - hia5
UseTags6xHis and Protein A/GExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-Rab6A
Plasmid#190171PurposeExpresses HaloTag-Rab6A in mammalian cells.DepositorInsertHaloTag-Rab6A
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-E2-Crimson
Plasmid#197196PurposeExpresses the protein of E2-Crimson in mammalian cellsDepositorInsertE2-Crimson
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor-tBFP-NLS-Neo
Plasmid#80766PurposeCustomizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.DepositorTypeEmpty backboneUseCRISPRTagsTagBFP2-3×NLSExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSPTg.T2FpAXK (#52)
Plasmid#35962DepositorInsertsTie2 promoter
SV40 pA
Tie2 enhancer
UseTagsExpressionMutationPromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
eeBxb1_IVT
Plasmid#222342PurposePlasmid for evolved and engineered Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTemplate for in vitro transcriptionTagsExpressionMutationPromoterT7 (inactivated)Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEa
Plasmid#193782PurposeExpress TadCBEa (with SpCas9) in mammalian cellsDepositorInsertTadA-CDa-SpCas9D10A
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
PH-Akt(R25C)-GFP
Plasmid#51466PurposeNon-PIP3 binding control for PH-Btk-GFPDepositorInsertAKT (aa 1–164)
UseTagsEGFPExpressionMammalianMutationR25CPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only