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pHR-mCherry-p2A-LIC1 G domain

Plasmid

#74602

Purpose

expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF1-ADAM29 (wt)

Plasmid

#31143

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM29 (ADAM29 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

WT

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-lox-ERAS

Plasmid

#52417

Purpose

Doxycycline inducible lentiviral vector of human ERAS cDNA with excisable insert

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

ERAS (ERAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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NEK2 gRNA (BRDN0001145920)

Plasmid

#76216

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK2 (NEK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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sgMLST8_2

Plasmid

#124906

Purpose

Targeting human MLST8 gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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DCLK1 gRNA (BRDN0001145902)

Plasmid

#76746

Purpose

3rd generation lentiviral gRNA plasmid targeting human DCLK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DCLK1 (DCLK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_TARBP2

Plasmid

#106119

Purpose

Lentiviral expression of TARBP2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TARBP2 (TARBP2 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Expression

Mutation

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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RRM1 E2.3 gRNA

Plasmid

#90881

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM1 (Guide Designation E2.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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JAK1 gRNA (BRDN0001145887)

Plasmid

#76392

Purpose

3rd generation lentiviral gRNA plasmid targeting human JAK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

JAK1 (JAK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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JAK1 gRNA (BRDN0001144778)

Plasmid

#76393

Purpose

3rd generation lentiviral gRNA plasmid targeting human JAK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

JAK1 (JAK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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JAK1 gRNA (BRDN0001149025)

Plasmid

#76395

Purpose

3rd generation lentiviral gRNA plasmid targeting human JAK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

JAK1 (JAK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shDnd1-No7

Plasmid

#70068

Purpose

TRC lentiviral vector for shRNA against mouse Dnd1 #7

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only
CCL2 gRNA (BRDN0001147096)

Plasmid

#77868

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCL2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CCL2 gRNA (CCL2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP4K4 gRNA (BRDN0001148610)

Plasmid

#76263

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K4 (MAP4K4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP4K4 gRNA (BRDN0001162594)

Plasmid

#76264

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K4 (MAP4K4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TP53RK gRNA (BRDN0001146381)

Plasmid

#77701

Purpose

3rd generation lentiviral gRNA plasmid targeting human TP53RK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TP53RK (TP53RK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-TARDBP-G287A_VA

Plasmid

#141328

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-G287A in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-G287A (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Mutation

G287A

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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TNIK gRNA (BRDN0001146045)

Plasmid

#75849

Purpose

3rd generation lentiviral gRNA plasmid targeting human TNIK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TNIK (TNIK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TNIK gRNA (BRDN0001149026)

Plasmid

#75851

Purpose

3rd generation lentiviral gRNA plasmid targeting human TNIK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TNIK (TNIK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IRAK1 gRNA (BRDN0001145661)

Plasmid

#76420

Purpose

3rd generation lentiviral gRNA plasmid targeting human IRAK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IRAK1 (IRAK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge