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Showing: 10861 - 10880 of 18174 results

  7. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166698-plasmid-map-sequence-id-329407

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  8. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-329408

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  12. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

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  13. EF1a_Puro_Telo_v2

    Plasmid
    #195139
    Purpose
    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction
    Depositor
    Jason Sheltzer
    Article
    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11
    Insert
    Telomere (RTEL1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195139-plasmid-map-sequence-id-442720

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  14. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

    Plasmid
    #162279
    Purpose
    Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN2A Variant 1, stabilized (SCN2A Human)
    Use
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp4551.
    Promoter
    CMV
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162279-plasmid-map-sequence-id-321873

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Showing: 10861 - 10880 of 18174 results