-
Plasmid#70038PurposeBacterial expression of the S. epidermidis Cas10·Csm complexDepositorInsertCas10/Csm complex
UseTags6xHIS on N terminus of Csm2ExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
sAB-MBP
Plasmid#204736Purposesynthetic antigen-binding fragment specifically recognizing bacteria maltose-binding proteinDepositorInsertsynthetic antigen-binding fragment recognizing bacteria maltose-binding protein
UseTagsExpressionMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Vinculin-N-21
Plasmid#55160PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertVinculin (VCL Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.21_PB-UGCG-PGK-Blast
Plasmid#205566PurposeExpress human UGCGDepositorInsertUGCG (UGCG Human)
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1510
Plasmid#91036PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSPDepositorInsertCsy4-P2A-TaCas9
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTD-NStrepHis
Plasmid#45936Purposeuse to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tagDepositorTypeEmpty backboneUseTagsHIS-6-tag and Twin-Strep-tagExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
mEos4b-Lifeact-7
Plasmid#54813PurposeLocalization: Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneUseTagsmEos4b-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCR1068
Plasmid#111092PurposeBFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationBlpI site disruptedPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
SBE-NLS-mCherry PGK-rtTA3
Plasmid#195332PurposeLentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3DepositorInsertsNLS-mCherry
rtTA3
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhPGK and miniCMVAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1910
Plasmid#91041PurposeModule A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSPDepositorInsertTREX2-P2A-TaCas9
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-oct4
Plasmid#20323DepositorInsertoct4 (Pou5f1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-YU2gp160
Plasmid#100927PurposeMammalian expression plasmid for Env from the YU-2 HIV-1 isolateDepositorInsertHIV-1 (YU2) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDFBB-eGFP
Plasmid#32550DepositorInsertegfp (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RosaL6 ELD mutations
Plasmid#37198DepositorInsertFokI (fused to Rosa L6 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs L6, and SV40 NLSExpressionMammalianMutationQ486E, I499L and N496DPromoterCMVAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
V51 pIRESpuro-GLUE (pGLUE)
Plasmid#15100Purposetandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tagsDepositorTypeEmpty backboneUseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
opto-Rac1
Plasmid#135396PurposeExpresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.DepositorInsertopto-Rac1
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNup133-Ypet
Plasmid#98283Purposemammalian expression of Nup133-YpetDepositorInsertNup133 (NUP133 Human)
UseTagsYPetExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
hRIP1 HA GFP K45A
Plasmid#41389DepositorInsertRIP1 (RIPK1 Human)
UseTagsGFP and HAExpressionMammalianMutationKinase Domain Mutant (K45A)PromoterCMVAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only