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Plasmid#225131PurposePhage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)DepositorInsertgp1 (A11V)
UseTagsHis-tagExpressionBacterialMutationAlanine 11 to ValinePromoterT7 promoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1561
Plasmid#218626PurposeExpress geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimerDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_35S-eGFP-T35S (GB4279)
Plasmid#225434PurposeTranscriptional unit for constitutive expression of the enhanced GFP.DepositorInsert35S:eGFP:T35S
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIND4-RC-M210TAG-A7
Plasmid#223015PurposeExpresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for 3-nitrotyrosineDepositorInsertspuhA
pufL
pufM
A7 RS
pylT tRNA
UseTags6xHisExpressionMutationchanged Tyr M210 to amber stop codon (TAG) for am…PromoterrrnBAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[]:mCherry-I{PIV-180}:NosT
Plasmid#212179PurposeThis binary vector expresses mCherry containing the PIV intron (180bp into the gene) with the truncated 35sCAMV promoterDepositorInsertmCherry-I{PIV-180}
UseTagsExpressionPlantMutationTruncated mCherry, missing the first 9 amino acid…Promotertruncated 35sCaMVAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/MtHp:nnLuz-I{PIV-216}:NosT
Plasmid#212192PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the strong Medicago trunculata MtHP promoter and UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…PromoterMedicago trunculata MtHP promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/SCBV:nnLuz-I{PIV-216}:NosT
Plasmid#212190PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the Sugarcane bacilliform virus (SCBV) promoter and UTRDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…PromoterSugarcane Bacilliform VirusAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_TetR
Plasmid#213847PurposeFacilitates genomic integration of tetR expression cassette.DepositorInsertTetR
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pET41-FeoB-OL2/4sub
Plasmid#216746PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL3/4sub
Plasmid#216747PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL3 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL5/4sub
Plasmid#216748PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL5 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PARP1-c009
Plasmid#216872PurposeBacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusionDepositorInsertPARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 FLAG-APEX2-P4C-IRES-mNeonGreen
Plasmid#218645PurposeGateway shuttling vector for APEX2-tagged P4C, a P4C biosensorDepositorInsertSidC(P4C)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His
Plasmid#218198PurposeBacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOMDepositorInsertEndoCOM
UseTags3C-CBD-HisExpressionBacterialMutationcodon optimized Ecoli, residues 19-315PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-NHSTIIT-GG
Plasmid#215789PurposeCloning of target gene with N terminal His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-GST-TEV-GG
Plasmid#215795PurposeCloning of target gene with N terminal GST tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-CT-sfGFP-HN-GG
Plasmid#215803PurposeCloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-CTH10STII-GG
Plasmid#215804PurposeCloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-CdRdhA
Plasmid#217824PurposeExpresses N-His-CdRdhA in E. coliDepositorInsertN-His-CdRhdA
UseTagsHis6xExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only