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Plasmid#213969PurposeAAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinaseDepositorInsertShank3 sgRNA
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA
Plasmid#154868PurposeHuman synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherryDepositorHas ServiceAAV Retrograde and AAV8InserthM3D(Gq)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_eCB2.0
Plasmid#164604PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in neuronsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterhSynAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP7SA11
Plasmid#89167PurposeExpress Rotavirus SA11 VP7 in mammalian cellsDepositorInsertRotavirus SA11 VP7
UseTags*see belowExpressionMammalianMutationPromoterT7 promotorAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
Plasmid#134868PurposeExpresses EGFP-tagged lysosomal markerDepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-Sox9
Plasmid#36979DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-WT-Hu1
Plasmid#177937PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN479
Plasmid#86234PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locusDepositorInsertspCas9-nickase and sgRNA against mouse rosa26 locus
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NLS-HA-TurboID
Plasmid#215075PurposeExpresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.DepositorInsertTurboID (birA )
UseLentiviralTagsNLS-HAExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
gTBEv3
Plasmid#220619Purposevector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorInserthUNG2-nSpCas9
UseTagsExpressionMammalianMutationhUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…PromoterhU6, EF1aAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-GFP
Plasmid#159654PurposeEmpty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPTbsr
Plasmid#117696PurposeBlasticidin-S resistance Vector for Phaeodactylum tricornutumDepositorTypeEmpty backboneUseVector for phaeodactylum tricornutumTagsExpressionMutationPromoterfcpBAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG_iPE-N
Plasmid#214734Purposeencodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoterDepositorInsertCAG_iPE-N_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP6SA11
Plasmid#89166PurposeExpress Rotavirus SA11 VP6 in mammalian cellsDepositorInsertRotavirus SA11 VP6
UseTagsExpressionMammalianMutationPromoterT7 promotorAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti_DCLK1
Plasmid#163625PurposeExpresses DCLK1DepositorInsertDCLK1 (DCLK1 Human)
UseLentiviralTagsV5ExpressionMutationPromotersv40Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ARAF
Plasmid#51076Purposemammalian expression of wt ARAFDepositorInsertARAF (ARAF Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_EGFP_m6A_Tracer
Plasmid#139403Purposein vitro transcription for mRNADepositorInsertm6A-Tracer
UseIn vitro transcriptionTagsEGFPExpressionMutationPromoterT3Available sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-ER
Plasmid#36204PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)DepositorInsertER-mTurquoise2
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-NbV5
Plasmid#201490PurposeNanoBit detection of V5-tagged proteinDepositorInsertHiBiT-NbV5
UseTagsHiBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(N3)-PP1beta
Plasmid#44223DepositorInsertPP1B (PPP1CB Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only