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Plasmid#176486Purposemouse Wnt1 cDNA in TetO backboneDepositorInsertmouse Wnt1 cDNA (Wnt1 Mouse)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJ841
Plasmid#39318DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …PromoterT7Available sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170809PurposeLentiviral vector to co-express an spsgRNA with NLS-mNeonGreenDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SBFP2-anti-AlfaTag nanobody
Plasmid#171826PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertSBFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5
Plasmid#126949PurposeBacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS478
Plasmid#89066PurposeKanMX Z3EV promoter cassetteDepositorInsertZ3EV promoter cassette
UseYeast integration vectorTagsExpressionMutationyeast Gal1 promoter modified with multiple Zif268…PromoterZ3EV promoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETcLIC-GFP
Plasmid#183514PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coliDepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTcI39-switch
Plasmid#195557PurposeTemperature state switch expressing low-temperature mWasabi and high-temperature mRFP1DepositorInsertsmRFP1
mWasabi
TcI39
UseTags6x His and AANDENYADAS degradation tagExpressionBacterialMutationA67TPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pBbB8k-csg-NbGFP
Plasmid#166858PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.DepositorInsertcsgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RaACR_687-EYFP
Plasmid#111077PurposeAnion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cellsDepositorInsertAnion channelrhodopsin RaACR_687
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTA7002-avrPto
Plasmid#49156PurposePlant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPtoDepositorInsertavrPto
UsePlant transformation binary vectorTagsExpressionMutationPromoterdexamethasone inducibleAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
BSA1_His
Plasmid#202766Purposebovine serum albumin design BSA1, in pET29b with C-terminal 6His-tagDepositorInsertBSA1
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 C506S
Plasmid#78584PurposeExpression flag-USP19 C506S in mammalian cellsDepositorInsertflag-USP19 C506S (USP19 Human)
UseTagsflagExpressionMammalianMutationmutate 506 cysteine residue to serinePromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMBP-SpyCatcher
Plasmid#79053PurposeExpresses maltose binding protein (MBP) SpyCatcher fusionDepositorInsertMBP-SpyCatcher
UseTags6xHis and Maltose binding proteinExpressionBacterialMutationDerived from pDEST14-SpyCatcher (Plasmid #35044)…PromoterT7Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
KA0637_pPBCAG-rtTAM2-IN
Plasmid#124166PurposepiggyBac-based expression of rtTA-M2DepositorInsertrtTAM2-IRES-Neo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1hWT
Plasmid#35027DepositorInsertFEN1 (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/1-CAG-Flex-FlpO-WPRE
Plasmid#171690PurposeCre-dependent expression of Flp-recombinaseDepositorInsertFlip-recombinase
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
USP19 sgRNA2
Plasmid#78586Purposedelete USP19 gene in human cellDepositorInsertUSP19 sgRNA2 (USP19 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only