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Plasmid#127713PurposeLevel 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only
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pCAG-LplA(AILR,36)-NRX3b D137A
Plasmid#43920DepositorInsertAILR-LplA36-NRX3b (lplA Synthetic, Human)
UseTags2XHAExpressionMammalianMutationD137APromoterChicken Beta ActinAvailable sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12m
Plasmid#69943PurposepSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanRDepositorInsertpSCKtheoRaj12m
UseSynthetic Biology; Psc101 origin of replicationTagsExpressionMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX2-AS-short-FlagTag (pcDNA3.1+)
Plasmid#75202PurposeMammalian expression of SLC24A2DepositorInsertSLC24A2 (SLC24A2 Human)
UseTagsFLAGExpressionMammalianMutationalternatively spliced exon absentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
Plasmid#209728PurposeExpresses cytosolic high affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP
Plasmid#226515PurposeMamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFPDepositorInsertCox8a(4x)-SNAP-tag2-T2A-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP
Plasmid#226516PurposeMamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFPDepositorInsertTOMM20-SNAP-tag2-T2A-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SC
Plasmid#224552PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsTwin-Strep-HAExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SN
Plasmid#224551PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsHA-Twin-StrepExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2861 (pSpy1C-PgyrA_ffluc)
Plasmid#218511Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPgyrAAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3105 (pSpy1C-PxylS2_ffluc)
Plasmid#218512Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPxylS2Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV110
Plasmid#215340PurposeExpresses sfGFP under induction with the Acrylic Acid Marionette Cassette (acuRAM + PAcu-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPAcuAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV101
Plasmid#215307PurposeExpresses sfGFP under induction with the Vanillic Acid Marionette Cassette (VanRAM + PVanCC-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPVanCCAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-P2A-3xFLAG-hEEA1 HDR template
Plasmid#214989PurposeEndogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.DepositorInsertEEA1 (EEA1 Human)
UseCRISPRTags3xFLAGExpressionMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-P2A-3xFLAG-msEEA1 HDR template
Plasmid#214990PurposeEndogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.DepositorInsertEEA1 (Eea1 Mouse)
UseCRISPRTags3xFLAGExpressionMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav-RA
Plasmid#209881PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1DepositorInsertddRFP A
UseTagsCAVIN1ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo40
Plasmid#204015PurposeCloning plasmid containing the mCherry insert with a chloramphenicol resistance markerDepositorInsertmCherry fluorescent protein
UseTagsExpressionPlantMutationPromoterN/AAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo41
Plasmid#204016PurposeCloning plasmid containing the BFP insert with a chloramphenicol resistance markerDepositorInsertBlue fluorescent protein
UseTagsExpressionPlantMutationPromoterN/AAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo42
Plasmid#204017PurposeCloning plasmid containing the YFP insert with a chloramphenicol resistance markerDepositorInsertYellow fluorescent protein
UseTagsExpressionPlantMutationPromoterN/AAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo5
Plasmid#203956PurposeCloning plasmid containing the chloramphenicol resistance gene insertDepositorInsertChloramphenicol Resistance Gene
UseTagsExpressionPlantMutationPromoterCAT promoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only