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pscALPS-SIV-SYK vpr

Plasmid

#115821

Purpose

Encodes codon optimized 3xFLAG-SIVSYK Vpr (L06042) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSYK Vpr (L06042)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-TAL vpr

Plasmid

#115820

Purpose

Encodes codon optimized 3xFLAG-SIVTAL Vpr (AM182197) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVTAL Vpr (AM182197)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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MASTL F3.1 gRNA

Plasmid

#90752

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MASTL (Guide Designation F3.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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DYRK1A gRNA (BRDN0001145031)

Plasmid

#76755

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1A (DYRK1A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NTRK1 gRNA (BRDN0001145717)

Plasmid

#75964

Purpose

3rd generation lentiviral gRNA plasmid targeting human NTRK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NTRK1 (NTRK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SASS6 G1.3 gRNA

Plasmid

#90884

Purpose

3rd generation lentiviral gRNA plasmid targeting human SASS6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SASS6 (Guide Designation G1.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001148101)

Plasmid

#80212

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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NEK4 gRNA (BRDN0001148553)

Plasmid

#77711

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK4 (NEK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERBB4 gRNA (BRDN0001162324)

Plasmid

#76198

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB4 (ERBB4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A IRES GFP

Plasmid

#133818

Purpose

Encodes the C2A domain of synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

Promoter

hSyn (human synapsin I promoter)

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSN20

Plasmid

#100105

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'medium' VP16 domain (POU5F1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-p110 CUX1

Plasmid

#100816

Purpose

Gateway lentiviral vector expressing p100 CUX1 (amino acids 747-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

Depositor

Alain Nepveu

Article

Vadnais et al BMC Genomics. 2013 Apr 16;14:258. doi: 1

Insert

Hs CUX1 amino acids 747-1505 of P39880 (CUX1 Human)

Use

Lentiviral

Tags

HA and myc

Expression

Mammalian

Mutation

contains amino acids 747-1505

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

Plasmid

#79383

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Zhang 2.0 + scaffold

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-2

Plasmid

#92157

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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TKFC gRNA (BRDN0001144729)

Plasmid

#75783

Purpose

3rd generation lentiviral gRNA plasmid targeting human TKFC

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TKFC (DAK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TKFC gRNA (BRDN0001146826)

Plasmid

#75784

Purpose

3rd generation lentiviral gRNA plasmid targeting human TKFC

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TKFC (DAK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PBK gRNA (BRDN0001145450)

Plasmid

#75572

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PBK (PBK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PBK gRNA (BRDN0001147308)

Plasmid

#75574

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PBK (PBK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC1orf27-1

Plasmid

#86130

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C1orf27

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 1 targeting C1orf27 (C1orf27 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG-RAB35 S22N

Plasmid

#107106

Purpose

Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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