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pY71-PT7-RiboJ-sfGFP-MGapt

Plasmid

#129119

Purpose

Expresses a fluorescent reporter for the quantification of cell-free protein expression.

Depositor

Matthew Lux

Article

Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

Insert

Superfolder GFP with Malachite Green aptamer

Use

Synthetic Biology

Tags

His6 and RiboJ Insulator

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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XE158 GFP-delta LZ-XDpr-CS2+

Plasmid

#16764

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

delta LZ-XDpr (dact1-b Frog)

Use

Xenopus expression

Tags

gfp

Expression

Mutation

XDpr is truncated to delete the first 129 amino a…

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

Plasmid

#72234

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

ADE3p

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pKEW321-MbCas12a-RFP-GFP-array

Plasmid

#115661

Purpose

Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

GFP and RFP array for MbCas12

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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78_pAAV-ProA27-CatCh-GFP-WPRE

Plasmid

#125910

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA27

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCALNL-eGFP-NT1-6-gix-6-NT1

Plasmid

#81204

Purpose

contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCBa

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcna1a-IRES-EGFP

Plasmid

#164965

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcna1a (kcna1a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

actinb (actb2) promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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45_pAAV-ProC10-CatCh-GFP-WPRE

Plasmid

#125945

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC10

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDUAL-Pnmt1-Ost4-GFP-MTn

Plasmid

#154369

Purpose

Yeast expression of Ost4

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Ost4 (ost4 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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PB CAG-L-bpA-2xSV40pA-L-tdGFP-Pten_shRNAmir

Plasmid

#178044

Purpose

CAG tdGFP Pten shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

Depositor

Mario Capecchi

Insert

Pten_1523 shRNAmir

Use

Cre/Lox and RNAi; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-CLASP2 512-650 9xS/A

Plasmid

#24410

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (512-650) 9xS/A (CLASP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant that c…

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

Plasmid

#177183

Purpose

plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

sgRNA targeting the PEAR-GFP plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B IRES GFP

Plasmid

#133817

Purpose

Encodes the C2B domain of synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

Promoter

hSyn (human synapsin I promoter)

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDestTol2-pax3a-kcnj13-IRES-EGFP

Plasmid

#164950

Purpose

Tol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

pax3a promoter -5.4k

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-IRES-EGFP

Plasmid

#164941

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

actinb (actb2) promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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193_pAAV-ProA35-CatCh-GFP-WPRE

Plasmid

#125918

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA35

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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105_pAAV-ProC16-CatCh-GFP-WPRE

Plasmid

#125951

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC16

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/PH* (W495L) uGFP

Plasmid

#14564

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

PH* (W495L)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
Sox2-SE-RGM-eGFP targeting vector

Plasmid

#133045

Purpose

Targeting vector of RGM-eGFP with homologous arms around Sox2 super-enhancer DMR

Depositor

Rudolf Jaenisch

Article

Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

Insert

Sox2-SE-5arm-RGM-Sox2-SE-3arm

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Drd1a->M71-IRES-tauGFP ACNF TV

Plasmid

#105073

Purpose

Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassette

Depositor

Peter Mombaerts

Article

Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.

Insert

Drd1a->M71-IRES-tauGFP-ACNF (Drd1, Olfr151 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Drd1a coding sequence followed by IRES-tauGFP ACNF

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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