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Plasmid#69548PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only
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pNeoXTR f2
Plasmid#69159Purposemouse targeting vectorDepositorInsertsDTA
NeoR
GFP
AmpR
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBCL2-FL(pA)
Plasmid#42594DepositorInsertBCL2 5'UTR - firefly luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-IndHom-Tol2-TetR-cryaa-dsRed
Plasmid#73204PurposeBAC targeting cassette for pIndigoBAC-536 with tetR selection marker and lens-specific transgenesis marker dsRedDepositorInsertdsRed
UseTagsExpressionMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBACgus_Sumostar/LRRK2 1867-2161_T2031E
Plasmid#40395DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2161, T2031EPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHyVec12
Plasmid#51851PurposeExpression in Hydra for RNAiDepositorInsertsGFP hairpin
DsRed2
UseRNAi; Hydra expressionTagsExpressionMutationPromoterActinAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNR64
Plasmid#79540PurposeInput plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.DepositorInsertsTP901
BxbI
UseTagsHis tag and LAA degradation tagExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR1055
Plasmid#71823PurposeExpress Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQP-NiRISB
Plasmid#138977PurposeLight-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)DepositorInsertNcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLN593 (FuGW-S(MAFK)p-mKate2)
Plasmid#105205Purposesynthetic promoter MAFK drives mKate2 expressionDepositorInsertS(MAFK)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p817-mgammaFcry-EGFP
Plasmid#23156DepositorInsertmouse gammaF crystallin promoter:EGFP (eGFP mouse gammaF promoter:EGFP, Mouse)
UseMulticloning site flanked by i-scei sitesTagsExpressionMutationmouse gammaF crystallin promoter (-225 - +73) pre…PromoterAvailable sinceApril 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-CaURA3
Plasmid#44881PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with CaUra3 selectionDepositorInsertyomKO2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTK101
Plasmid#59561PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4DepositorInsertSGP(14) mKate-G8-L7Ae-PEST 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PP363
Plasmid#78984Purpose255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.DepositorInsertsrHGH
IFNa2b
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
CENP-B mCherry PP1 gamma
Plasmid#45221DepositorInsertCENP-B mCherry PP1 gamma
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-IndHom-Tol2-FRT-GalK-cryaa-dsRed
Plasmid#73203PurposeBAC targeting cassette for pIndigoBAC-536 with GalK selection marker and lens-specific transgenesis marker dsRedDepositorInsertdsRed
UseTagsExpressionMutationPromoterAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA1-lenti
Plasmid#115662PurposeExpresses AcrVA1 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA1
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a promoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
LacI INCENP
Plasmid#45236PurposeTarget INCENP to lac operator sequenceDepositorInsertLacI, INCENP-Δcen, mCherry (lacI )
UseTagsLacI (lac repressor protein) and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)Promotert7Available sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
p15aC-4D1D
Plasmid#107864PurposeBacterial expression of PIS and PI4KDepositorInsertsPIS
phosphatidylinositol 4-kinase β
UseTagsmycExpressionBacterialMutationPromoterproDAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVD_85
Plasmid#149604Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylRDepositorInsertXylR
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterTEF1Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only