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Plasmid#213837PurposeFacilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF1_mNeonGreen
Plasmid#213840PurposeFacilitates genomic integration of the reporter with upstream binding sites for ZF-1DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF2_mNeonGreen
Plasmid#213841PurposeFacilitates genomic integration of the reporter with upstream binding sites for ZF-2DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pStorage_Ptet-1_ZF1
Plasmid#213844PurposeCombiGEM storage plasmid for ZF-1 CR fusions.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pStorage_Ptet-2_ZF2
Plasmid#213845PurposeCombiGEM storage plasmid for ZF-2 CR fusions.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEU
Plasmid#213846PurposeLEU integrating plasmid backbone used to create all of the reporter plasmids.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_TetR
Plasmid#213847PurposeFacilitates genomic integration of tetR expression cassette.DepositorInsertTetR
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CdRdhA
Plasmid#217824PurposeExpresses N-His-CdRdhA in E. coliDepositorInsertN-His-CdRhdA
UseTagsHis6xExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-RdhC1
Plasmid#217822PurposeExpresses HisSUMO-RdhC1 in E. coliDepositorInsertRdhC1
UseUnspecifiedTagsHis10xSUMOExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-RdhF3
Plasmid#217823PurposeExpresses HisSUMO-RdhF3 in E. coliDepositorInsertRdhF3
UseTagsHis10xSUMOExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DhFld
Plasmid#217821PurposeExpresses N-His-DhFld in E. coliDepositorInsertDhFld
UseTagsHis6xExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
PAS_HeSAHS4
Plasmid#215648PurposeExpresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coliDepositorInsertSAHS4
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PAS_RvSAHS4
Plasmid#215646PurposeExpresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coliDepositorInsertSAHS4
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[]:nnLuz-I{PIV-216}:NosT
Plasmid#212187PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the truncated 35sCAMV promoterDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-mCherry-WPRE-UbC-MCS
Plasmid#211801PurposeLentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoterDepositorInsertsmCherry
multiple cloning site
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS_3MYC_PAC
Plasmid#122566PurposeVector for endogenously tagging Giardia genes with a triple Myc tag (Puromycin selection)DepositorTypeEmpty backboneUseTags3x c-Myc tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS_3HA_NEO
Plasmid#122565PurposeVector for endogenously tagging Giardia genes with a triple HA tag (G418 selection)DepositorTypeEmpty backboneUseTagshemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Toxoplasma gondii (delta prenylation motif)
Plasmid#170789PurposeExpresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)DepositorInsertToxoplasma gondii PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-RLuc-5BoxB_C
Plasmid#146091PurposeMammalian Expression of 5BoxBDepositorInsert5BoxB
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHS29
Plasmid#171205Purposebacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motifDepositorInsertmeg-3 (meg-3 Nematode)
UseTags6xHISExpressionBacterialMutationF705A,Y708A,Y713A, N725APromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only