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Plasmid#16835DepositorInsertDelta beta-catenin GFP (ctnnb1.L Frog)
UseXenopus expressionTagsGFPExpressionMutationThese PCR fragments have the first 138 bp after t…PromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-CaMKKbeta S129D, S133D, S137D
Plasmid#32455DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseTagsFLAG and GFPExpressionMammalianMutationS129D, S133D, S137DPromoterCMVAvailable sinceMay 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-AICD-NLS-IRES-hrGFP
Plasmid#107543PurposeAAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFPDepositorInsertAICD-NLS (APP Human)
UseAAVTagsExpressionMutationNonePromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-splitTVA-EGFP
Plasmid#59332PurposeExpresses splitTVA-P2A-EGFPDepositorInsertsplitTVA950-P2A-EGFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3XFLAG-EGFP-NLS hygro
Plasmid#87781PurposeInducible expression of EGFP with a C-terminal NLS sequenceDepositorInserteGFP
UseLentiviralTags3xFLAG and NLSExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-UBE3BΔHECT-copGFP-Puro
Plasmid#107343PurposeExpresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.DepositorInsertUBE3B(ΔHECT)-copGFP (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationdeletion of HECT domain (757-end)PromoterCMVAvailable sinceJune 26, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEM-myr-eGFP-SV40pA-FKF
Plasmid#119866PurposeDonor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p144 pSV-EGFP/beta-gal
Plasmid#8386DepositorInsertfloxed EGFP
UseCre/Lox; Beta-galTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-mCherry-P/PM,4A5C6G7G-GFP
Plasmid#80914PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationPromoterP4A5C6G7G and PM,4A5C6G7GAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
KZ901: pMVP (L3-L2) P2A-eGFP + WPRE
Plasmid#121772PurposepMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-eGFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
VV221: sdChR(C138S)-TS-eGFP-ER in fck
Plasmid#58513PurposeExpresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoterDepositorInsertsdChR(C138S)-TS-eGFP-ER
UseLentiviralTagseGFPExpressionMammalianMutationchanged Cysteine 138 to SerinePromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-PLtetO1-deGFP-T500
Plasmid#45392DepositorInsertdeGFP
UseTagsExpressionBacterialMutationPromoterPL-tetO1Available sinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
BE3(E63Q)-P2A-EGFP (pRZ189)
Plasmid#123613PurposeCAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).DepositorInsertBE3(E63Q)-P2A-EGFP
UseTagsExpressionMammalianMutationE63Q in rAPOBEC1, D10A in SpCas9PromoterCAGAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain
Plasmid#58725PurposeExpresses GFP-Tagged CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαWT-IRES-EGFP
Plasmid#183456PurposeReplacement of endogenous rat PKA-RIIα subunits with wild-type RIIαDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralTagsExpressionMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/UAA-mEGFP-IRES-mCherry
Plasmid#49225PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Suz12-F2-GFP
Plasmid#91882PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12DepositorInsertgRNA targeting Suz12 (Suz12 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RNF138-delta 158-180
Plasmid#78923PurposeMammalian expression of RNF138 with delta 158-180 and an EGFP fusionDepositorInsertRNF138 (RNF138 Human)
UseTagsEGFPExpressionMammalianMutationdelta 159-180PromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RNF138-delta 189-215
Plasmid#78924PurposeMammalian expression of RNF138 with delta 189-215 and an EGFP fusionDepositorInsertRNF138 (RNF138 Human)
UseTagsEGFPExpressionMammalianMutationdelta 189-215PromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT eGFP-RVFV NSs HhRz
Plasmid#91801PurposeExpresses a non-polyadenylated, translatable EGFP mRNA in DrosophilaDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only