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Plasmid#135463PurposeNeuronal expression of uFLARE protease componentDepositorInserteGFP-CaM-uTEV1Δ
UseAAVTagseGFP, V5ExpressionMammalianMutationS219V mutation improves stability and S153N impro…PromoterSynapsinAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCngA2-ISH
Plasmid#15855DepositorInsertCyclic Nucleotide Gated Channel Alpha 2 (Cnga2 Mouse)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCaSper4 cyto roGFP2-Orp1
Plasmid#64998PurposeExpression of cytosolic roGFP2-Orp1 under the alphaTub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmRuby2-CrowdingLinker-Clover
Plasmid#171058PurposeExpresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.DepositorInsertCrowdedness-sensitive linker domain
UseTagsmClover2 and mRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-b2AR-hLOV1-TEVcs-FLAG-GAL4-V5
Plasmid#104842Purposeb2AR-SPARK TF componentDepositorInserthLOV1-TEVcs-FLAG-GAL4-V5
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNM3749
Plasmid#154108PurposeloxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX2-AS-long-FlagTag (pcDNA3.1+)
Plasmid#75203PurposeMammalian expression of SLC24A2DepositorInsertSLC24A2 (SLC24A2 Human)
UseTagsFLAGExpressionMammalianMutationalternatively spliced exon presentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAT.011
Plasmid#119555PurposeSelf replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM321 - array co-injection fragment mosTI hygroR
Plasmid#159828PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertarray co-injection fragment mosTI hygroR
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
Plasmid#125233PurposeExpresses "sender" trans-cellular NanoLuc in mammalian cellsDepositorInsertgLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK088
Plasmid#171360PurposeAMA1 sequence as Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertAMA1 sequence (short), entry vector providing fungal replication
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a - CPX
Plasmid#89620Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertCytosolic Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pinco-mNrp1dCy
Plasmid#21936DepositorInsertneuropilin 1 (Nrp1 Mouse)
UseRetroviralTagsHA and HisExpressionMutationdeleted amino acids 884-923PromoterAvailable sinceNov. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2259 - Intron GG2 - 900 bp
Plasmid#159881PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG2 - 900 bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pinco-mNrp1dSEA
Plasmid#21935DepositorInsertneuropilin 1 (Nrp1 Mouse)
UseRetroviralTagsHA and HisExpressionMutationdeleted amino acids 920-923PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCoofy57
Plasmid#122009PurposeMammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis8 and Multi1: depending on the LP2 primer, eit…ExpressionMammalianMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-LplA(AILR,36)-NRX3b
Plasmid#43919DepositorInsertAILR-LplA36-NRX3b (lplA Synthetic, Human)
UseTags2XHAExpressionMammalianMutationPromoterChicken beta actinAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)
Plasmid#159886PurposeThree-fragment Gateway compatible promoter with PATCs for germline expression from arraysDepositorInsert[4-1] - Promoter - Psmu-1(no_atg)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)
Plasmid#159887PurposeThree-fragment Gateway compatible promoter with PATCs for germline expression from arraysDepositorInsert[4-1] - Promoter - Psmu-2 (no_start)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1359 - Intron GG2 - smu-1
Plasmid#159805PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG2 - smu-1
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only