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Plasmid#216348Purposetargets ECFP-RspANFAST to mitochondrial membraneDepositorInserttomm20-ECFP-RspANFAST
UseTagstomm20ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-mCherry
Plasmid#114013PurposeEmpty sgRNA expression vector with co-expression of mCherry reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisB-TRF2
Plasmid#50488PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 (TERF2 Human)
UseTags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 3-500PromoterpTrcAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCJ202
Plasmid#162675PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.DepositorInsertPETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
UseTagsHisExpressionBacterialMutationW159C, S238C; codon optimized for expression in E…PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
EB3-mNeonGreen
Plasmid#191761PurposeExpression of mNeonGreen fused to EB3DepositorInsertEB3-mNeonGreen (MAPRE3 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5059
Plasmid#198154Purposebacterial expression of steC::HaloTag fusionDepositorInsertPsteC steC::L16::HaloTag::HA
UseTagsHaloTag-HAExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC70207
Plasmid#209468PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU6Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt1
Plasmid#49186PurposecDNA of Akt1 with myristyolation signal in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationPromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
CENP-B mCherry
Plasmid#45219DepositorInsertCENP-B mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C-GFP
Plasmid#130975PurposeExpression of 6His-KIF1C-GFP in insect cells for protein purification.DepositorInsertKIF1C-GFP (KIF1C Human)
UseTags6His and GFPExpressionInsectMutation5 silent mutations that make this construct RNAi …PromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only