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pCas4_1_2_VA
Plasmid#186438PurposeEncodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-UPRT-T2A-RFP-IRES-CD
Plasmid#126677PurposeDoxycycline inducible expression of UPRT and CD along with reporter RFPDepositorInsertUPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase
Plasmid#163404PurposeExpresses BMPR-2 lacking the kinase domain in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe kinase domain (202-500 aa) is deletedPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJB6
Plasmid#177657PurposeRecombinant protein synthesis of human DNAJB6DepositorInsertDNAJB6 (DNAJB6 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-Puro-TDP-43-A382T
Plasmid#133756PurposeLentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMutationChanged alanine 382 to threoninePromoterCMVAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-RR
Plasmid#37276PurposeTALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)DepositorTypeEmpty backboneUseTagsC terminal Tal From pTAL3, FokI RR, HA, and N-ter…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
UseTags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Clathrin-15
Plasmid#58005PurposeLocalization: Clathrin Vesicles, Excitation: 555, Emission: 584DepositorInsertClathrin (CLTB Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-BR1
Plasmid#160333PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.DepositorInsertBR1
UseTags6xHis tagExpressionBacterialMutationPromoterT5Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREMI-ZA
Plasmid#183736PurposePlasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffiiDepositorInsertSh ble
UseRandom insertional gene deletion in komagataella …TagsExpressionMutationPromoterTEF1 promoterAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_WT
Plasmid#162579PurposeExpresses norovirus GI.1 VP1 protein in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGTM_COV2_NSP5_004_SUMO
Plasmid#190062PurposeExpresses SARS-CoV-2 3CLpro for the expression of the active mature proteaseDepositorInsert3CLpro (ORF1ab SARS-CoV-2)
UseTags6X-His-SumoExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDIRECT_21C
Plasmid#91130PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas-CmR(+)
Plasmid#113633PurposeChloramphenicol resistant derivative of pCas plasmidDepositorInsertChloramphenicol resistance gene
UseCRISPRTagsExpressionMutationPromoterPcatAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
CathepsinG-CD4d3+4-bio
Plasmid#73125PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding CathepsinG with rat CD4d3+4-bioDepositorInsertCathepsin G (CTSG Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Flex-mRuby3-2A
Plasmid#203846PurposeNeuron specific expression of a Cre-dependent mRuby3DepositorInsertmRuby3
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-SomArchon
Plasmid#126943PurposeCre-dependent expression of SomArchon under CAG promoterDepositorInsertSomArchon
UseAAVTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEFL EGFP-TEADi
Plasmid#140144PurposeTEAD inhibitor (TEADi): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal.DepositorInsertEGFP TEADi
UseTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only
