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Plasmid#25310PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTJP3:G508-L808:cH6 (TJP3 Human)
UseTagsHisExpressionBacterialMutationContains amino acids G508-L808PromoterAvailable sinceApril 4, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK652
Plasmid#72470PurposeProduces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)DepositorInsertacetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationMutated histidine-233 to glycinePromoterT7Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK288
Plasmid#71552PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with Tyr260>Ala mutant (AaAlr-Y260A)DepositorInsertalanine racemase
UseTagsExpressionBacterialMutationchanges tyrosine-260 to alaninePromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK301
Plasmid#71555PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with C-terminal His tag (AaAlrH6)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK559
Plasmid#71603PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tyrosine-…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK562
Plasmid#71604PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tyrosine-…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK604
Plasmid#71610PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, histidine…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK664
Plasmid#71613PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparagine, tyrosine-154 …PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK666
Plasmid#71615PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparagine, tryptophan-97…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK563
Plasmid#71689PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tyrosine-…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK508
Plasmid#71598PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp165>Phe mutant (AaPurE1fwf-H59N)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, histidine…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP-MAPK2 (3RP9)
Plasmid#32862PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK2
UseTagsHis tag with TEV cleavageExpressionBacterialMutationamino acids 123-548 only, P421LPromoterAvailable sinceJan. 10, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
TDRD7 (3RCO)
Plasmid#32051PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTDRD7 (TDRD7 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
SGF29 (3MEU)
Plasmid#25317PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29_6 (SGF29 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 115-293PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
SGF29 (3MEW)
Plasmid#25320PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29_23 (SGF29 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 129-291PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
SGF29 (3LX7)
Plasmid#25314PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29_3 (SGF29 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 138-291PromoterAvailable sinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
C6orf197
Plasmid#25258PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertC6orf197:M178-R505 (TBC1D22B Human)
UseTagsHisExpressionBacterialMutationContains amino acids M178-R505PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PLXND1 (3H6N)
Plasmid#25187PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPLXND1:A1553-D1656 (PLXND1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids A1553-D1656PromoterAvailable sinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
p6112 pSG5-HPV31-d3-12-E8^E2C(HA)
Plasmid#24743DepositorInsertHPV31 d3-12 E8^E2C (HA)
UseTagsHA tag in hinge regionExpressionMammalianMutationAmino acids 3-12 missing.PromoterAvailable sinceJune 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin WT
Plasmid#12647DepositorInsertUbiquitin - wild type (UBC Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only