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Plasmid#85779PurposeN-ICE plasmid pK3FS-CYC1DepositorInsertloxP-KanMX-loxP-CYC1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PonA-BI-Gl-WT-18xPP7-WT-24xMS2 (Switch)
Plasmid#189793PurposeExpress wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorInsertbeta-globin (Hbb Human, Mouse)
UseTagsExpressionMammalianMutationPromoterPonasterone A inducibleAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA107
Plasmid#59785Purposedrives ZIF-1 protein in C. elegans ubiquitouslyDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-EGFP100
Plasmid#46309DepositorInsertsp19
EGFP100 DNA-1
linker
EGFP100 DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E-CaaX(tH)
Plasmid#109539PurposeMultisite gateway vector for 3' tagging with the CaaX domain from HrasDepositorInsertCaaX domain from Hras
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEos2-CaURA3
Plasmid#44887PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with CaUra3 selectionDepositorInsertyomEos2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 bPAC cMyc S27A 2A tDimer
Plasmid#85398Purposehumanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent proteinDepositorInsertshumanized photoactivated adenylyl cyclase
red fluorescent protein
UseAAVTagscMyc TagExpressionMammalianMutationS27APromoterhuman Synapsin1 promotor and ribosomal skip seque…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP657-SpHis5
Plasmid#44846PurposeYeast tagging vector to create gene fusion with yeast optimized TagRF657 with SpHis5 selectionDepositorInsertyoTagRFP657
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQP-AR10
Plasmid#138980PurposeEndogenous RAS regulation using light-controlled intrabodyDepositorInsertBglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-Kan
Plasmid#44958PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with Kan selectionDepositorInsertyomKO2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVC-CRISPR
Plasmid#120369PurposePlasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing libraryDepositorInsertsCRISPR1
CRISPR2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP212-Lenti-1.3CaMKII-P-Intron-MCS-WPRE-pA
Plasmid#62931PurposepLenti vector backbone containing a 1.3CaMKII promoter followed by an intron followed by multiple cloning site(MCS) (reverse orientation).DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoter1.3αCaMKIIAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE
Plasmid#101101PurposeEncodes dCas9(N) fused to transmembrane tetherDepositorInsertTMt-dCas9(N)
UseCRISPR and Synthetic BiologyTagsHA and MycExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEV_S
Plasmid#100266PurposepDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coliDepositorInsertsUseSynthetic Biology; Expression, pbr322TagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Thy1.2-NLS_HA_Dre-P2A-BFP
Plasmid#51275PurposeCo-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassetteDepositorInsertNLS_HA_Dre-P2A-BFP
UseTagsExpressionMutationPromoterThy1.2Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_WT
Plasmid#175044PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationWT (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLSLZ.D.R
Plasmid#51510PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTC150
Plasmid#70014Purpose'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral RepliconDepositorInsertDonor + GVR
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7
Plasmid#46306DepositorInsertsp19
DNA-1 site
linker
DNA-2 site
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterTacAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPA-mCherry-SpHis5
Plasmid#44855PurposeYeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with SpHis5 selectionDepositorInsertyoPA-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only