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pmCellFree_KA1_SARS-COV-2_NSP3
Plasmid#169378PurposeCell-free/mammalian expression vector for SARS-COV-2 virus NSP3 with N-term eGFPDepositorInsertSARS-COV-2 _NSP3
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHD-w+
Plasmid#80927PurposeVector for generating dsDNA donors for HDR. Empty backbone containing a StuI restriction site and GMR-white for identifying backbone incorporation.DepositorInsertGMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoterGMR::hsp70Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pB-CAG-PE2-NatMX
Plasmid#158567PurposePiggybac vector that allows for the generation of PE2-expressing stable cell linesDepositorInsertPE2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-Pur LaG17-synNotch-Gal4VP64
Plasmid#158135PurposeConstitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backboneDepositorInsertLaG17-synNotch-Gal4VP64
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJF3H
Plasmid#63897PurposeFor phage display of cDNA librariesDepositorInsertcDNA cloning region
UsePhage displayTagsFosExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCM1.53
Plasmid#17250DepositorInsertGFP-no stop in pDONR201
UseGatewayTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
IDG_TMEM38B_OE_1
Plasmid#161658PurposeOverexpresses TMEM38BDepositorInsertTMEM38B (TMEM38B Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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p416-GPD-PRUNE-HA
Plasmid#183945PurposeExpresses human HA-tagged PRUNE from yeast GPD promoterDepositorInsertPRUNE (PRUNE1 Human)
UseTagsHAExpressionYeastMutationWTPromoterGPDAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExp-Trx
Plasmid#112577PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-TrxA and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCM1.57
Plasmid#17252DepositorInsertL3788 promoter NO ATG in pDONRP4P1R
UseGatewayTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EYFP-ccdB-G418
Plasmid#158550PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFRT-DestFLAGHA_RNAseH1
Plasmid#65783Purposeconstitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cellsDepositorInsertRNaseH1 (RNASEH1 Zebrafish, Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
