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Plasmid#50865PurposeExpresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationA675C C723S C724V A735C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V V729C (NT543)
Plasmid#50907PurposeExpresses human NKCC1 C723S C724V V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V V729C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V W733C (NT547)
Plasmid#50909PurposeExpresses human NKCC1 C723S C724V W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V W733C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V F728C (NT542)
Plasmid#50906PurposeExpresses human NKCC1 C723S C724V F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V F728C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V M727C (NT540)
Plasmid#50905PurposeExpresses human NKCC1 C723S C724V M727C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationC723S C724V M727C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.AEBP2
Plasmid#125165PurposeExpresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertZinc finger protein AEBP2 (AEBP2 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPR
Plasmid#111502PurposeExpresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].DepositorInsertZF(VEGFA)-StaPL(AI)-YFP-VPR
UseTagsExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-wt
Plasmid#184269PurposeExpresses shRNA resistant GFP-RepoManDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTags3xHA and GFPExpressionMammalianMutationchanged nucleotides 723-747, 1488-1509, 1524-1548…PromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII
Plasmid#217897PurposeInsect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tagDepositorUseTags2xSTIIExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1540
Plasmid#82433PurposePlasmid for expression of GST-tagged human-Nematostella GW182 11W11A chimera in mammalian cellsDepositorInserths-nvGW182 11W11A chimera
UseTagsGSTExpressionMammalianMutationaa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …PromoterEF1AAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 KSR2
Plasmid#25968DepositorInsertKinase suppressor of Ras 2 (Ksr2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV KSR2 IRES GFP
Plasmid#25969DepositorInsertKinase suppressor of Ras 2 (Ksr2 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPB-VPR-dSpCas9[StaPL(TI)]
Plasmid#111508PurposeExpresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].DepositorInsertVPR-dSpCas9[StaPL(TI)]
UseTagsExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, and S122…PromoterPGKAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry
Plasmid#226104PurposeStability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cellsDepositorInsertCOX8A (COX8A Human)
UseTagsGFPExpressionMammalianMutationencodes only for mitochondrial targeting sequence…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR-BLINK2
Plasmid#117075PurposeBLINK2 gene in pDONR vector for Gateway cloning systemDepositorInsertBLINK2
UseGateway cloning systemTagsExpressionMutationglutamine 112 changed into aspartate (original LO…PromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2
Plasmid#202762PurposeEncodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harborDepositorInsertNEUROG2 (NEUROG2 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-744pUC19-tNGFR-P2A-CD2
Plasmid#186118PurposeKnockin of truncated NGFR to target geneDepositorInsertCD2 (CD2 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tet-off TauRD (P301L/V337M)
Plasmid#188572PurposeDox-repressible expression of the Tau repeat domainDepositorInsertTau repeat domain (MAPT Human)
UseLentiviralTagsMycExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_Cas9-blast_GFP
Plasmid#162074Purpose3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistanceDepositorInsertsCas9-2a-blasticidin S deaminase
EGFP
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEF1a and sv40Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_Cas9-hygro_GFP
Plasmid#162075Purpose3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistanceDepositorInsertsCas9-2a-hygromycin B phosphotransferase
EGFP
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEF1a and sv40Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only