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Showing: 1061 - 1080 of 51885 results
  1. pBGE2.0

    Plasmid
    #165987
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.
    Depositor
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    tetracycline resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pAGE3.0

    Plasmid
    #165985
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Nm/Kan resistance.
    Depositor
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    neomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pSB61 - pL0_V2 (CDS1)

    Plasmid
    #123183
    Purpose
    Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005
    Insert
    Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
    Use
    Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pLV-EF1A-CD19.8H.8TM.28.BB.3z

    Plasmid
    #200672
    Purpose
    Modular CD8hinge-CD8transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Insert
    FMC63-CD8HD-CD8TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pLV-EF1A-CD19.8H.28TM.BB.3z

    Plasmid
    #200675
    Purpose
    Modular CD8hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Insert
    FMC63-CD8HD-CD28TM-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pLV-EF1A-CD19.8H.8TM.BB.28.3z

    Plasmid
    #200673
    Purpose
    Modular CD8hinge-CD8transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Insert
    FMC63-CD8HD-CD8TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pLV-EF1A-CD19.8H.28TM.BB.28.3z

    Plasmid
    #200677
    Purpose
    Modular CD8hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Insert
    FMC63-CD8HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pLV-EF1A-CD19.8H.28TM.28.BB.3z

    Plasmid
    #200676
    Purpose
    Modular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Insert
    FMC63-CD8HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Insert
    KRas c-term (KRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid
    #58492
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)
    Depositor
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    deleted amino acids P29 to D408 and bearing mutat…
    Promoter
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. MTST5

    Plasmid
    #86602
    Purpose
    Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
    Depositor
    Insert
    MTST5
    Use
    Internal standard reverse transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1061 - 1080 of 51885 results