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Plasmid#199369PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOstar-ALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pET26b-T4gp45
Plasmid#228204PurposeExpression in BL21(DE3) of WT T4 clampDepositorInsert45 (45 Escherichia phage T4)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaryP
Plasmid#18945DepositorInsertphiC31 attP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJF3H
Plasmid#63897PurposeFor phage display of cDNA librariesDepositorInsertcDNA cloning region
UsePhage displayTagsFosExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4-4T-1 BCP1 NTase
Plasmid#161812PurposeExpresses SMODS/CD-NTase from Bacillus phage Bcp1DepositorInsertphage BCP1 SMODS/CD-NTase enzyme
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTA-attP
Plasmid#18939DepositorInsertphiC31 attP
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pRVV601
Plasmid#87632PurposeRMCE Bxb1 attB-flanked vector.DepositorInsertBxb1 attB
UseRmceTagsExpressionMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
P4 cosmid
Plasmid#196335PurposeP4 cosmid with CRISPR-Cas9 systemDepositorInsertgenes from phage P4, CRISPR-Cas9 system
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR
Plasmid#99244PurposeFor autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorInsertphage lambda gene R (R phage lambda)
UseTagsExpressionBacterialMutationPromoterbeta-lactamase promoterAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVV651
Plasmid#87631PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorInsertBxb1 attP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNitET-SacB-kan
Plasmid#107692PurposeExpression of the phage Che9c RecET system for Recombineering in mycobacteriaDepositorInsertsChe9 phage RecET
PNit2-NitR
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-sfGFP
Plasmid#51562PurposeExpression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)DepositorInsertsuperfolder GFP
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRVV647
Plasmid#87630PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorInsertBxb1 attP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVV653
Plasmid#89474PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorInsertBxb1 attP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVV598
Plasmid#87629PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorInsertBxb1 attP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR (in lacZ deficient strain)
Plasmid#99245PurposeFor autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorInsertphage lambda gene R (R phage lambda)
UseTagsExpressionBacterialMutationPromoterbeta-lactamase promoterAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFd022.R2
Plasmid#137967PurposeExpresses Prochlorococcus phage P-SSM2 phage FdDepositorInsertProchlorococcus phage P-SSM2 ferredoxin
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJTA022
Plasmid#137975PurposeInucible expression of Prochlorococcus phage P-SSM2 phage Fd for purificationDepositorInsertProchlorococcus phage P-SSM2 ferredoxin
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11phiC31poly(A)
Plasmid#18942DepositorInsertphiC31 integrase (int Streptomyces phage phiC31)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only