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Plasmid#72824PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Neo
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAluYa5-neo-TET
Plasmid#51283Purposeexpresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertAluYa5
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoterupstream pol III enhancer sequence of the 7SL gen…Available sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-DEST
Plasmid#80490PurposeGateway Destination donor vector for AAVS1 targeting (puromycin selection)DepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK293 (mAID-mCherry2-Hygro)
Plasmid#72831PurposeC-terminal tagging with mAID-mCherry2 using CRISPR/Cas9DepositorInsertmAID-mCherry2-Hygro
UseCRISPRTagsmAID-mCherry2ExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
L1-neo-TET
Plasmid#51284Purposeexpresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassetteDepositorInsertL1RE1 (L1RE1 Human)
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS U6a gRNA
Plasmid#158961PurposegRNA backbone driven by zebrafish U6a promoterDepositorInsertszf U6a promoter
gRNA backbone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK291 (mAID-mClover-Bsr)
Plasmid#72829PurposeC-terminal tagging with mAID-mClover using CRISPR/Cas9DepositorInsertmAID-mClover-Bsr
UseCRISPRTagsmAID-mCloverExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Spy CCR5-pegRNA
Plasmid#169856PurposeSpyCas9-pegRNA for CCR5DepositorInsertSpy CCR5-pegRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Nst-MCS
Plasmid#80487Purposedonor vector for AAVS1 targeting (neomycin selection) with multiple cloning siteDepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK292 (mAID-mCherry2-NeoR)
Plasmid#72830PurposeC-terminal tagging with mAID-mCherry2 using CRISPR/Cas9DepositorInsertmAID-mCherry2-Neo
UseCRISPRTagsmAID-mCherry2ExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse c-Myc
Plasmid#14972DepositorInsertc-Myc (Myc Mouse)
UseTagsExpressionBacterialMutationNucleotides 2578-3294PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-IT6
Plasmid#66870PurposeInntag 6: a small self-structured epitope aimed at innocuous protein taggingDepositorInsertInntag 6
UseTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 EMX1-nicking sgRNA
Plasmid#169859PurposeSpyCas9 nicking sgRNA for EMX1DepositorInsertSpyCas9 EMX1-nicking sgRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Spy EMX1-pegRNA
Plasmid#169855PurposeSpyCas9-pegRNA for EMX1DepositorInsertSpy EMX1-pegRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
PDX1-2A-eGFP
Plasmid#66964PurposePDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locusDepositorInsertpancreatic and duodenal homeobox 1 (PDX1 Human)
UseUnspecifiedTags2A-eGFPExpressionMutationPromoterAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Δrev
Plasmid#101348PurposeAll mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta revPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSSK WT p85 alpha flag
Plasmid#13429DepositorInsertp85 alpha (PIK3R1 Human)
UseTagsflagExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPEE47
Plasmid#139426PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE46
Plasmid#139425PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only