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pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM
Plasmid#183077PurposePlasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.DepositorInsertCas12c tracrRNA and crRNA ending with a HDV ribozyme
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA4-lenti
Plasmid#115663PurposeExpresses AcrVA4 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA4
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEF-1a promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti
Plasmid#115795PurposeExpresses AcrIIA4 in human cells, contained in a lentiviral vector.DepositorInsertAcrIIA4
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEF-1a promoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLBH546_Tet-Cas14b1Locus
Plasmid#112503PurposeExpresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14b1
UseCRISPRTagsExpressionBacterialMutationPromoterTetAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBA1015
Plasmid#189589PurposeT7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)DepositorInsertT7gp1.7 Locus 250bp Homology Arms
UseSynthetic BiologyTagsExpressionBacterialMutationEncodes for a full deletion of T7gp1.7PromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBH547_Tet-Cas14b2Locus
Plasmid#112504PurposeExpresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14b2
UseCRISPRTagsExpressionBacterialMutationPromoterTetAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cas12c-sgRNA_BbsI_CAM
Plasmid#183221PurposeCas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertCas12c single guide RNA containing BbsI sites in spacer sequence
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA630
Plasmid#186241PurposeRfxCas13d Negative Control (RFP targeting)DepositorInsertpTet-RfxCas13d + crRNA negative control
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
T1P/pUBQ10(mTurq->mScarlet)
Plasmid#195945PurposePhiC31 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_PhiC31-pUBQ10-attB_PhiC31-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a H759A
Plasmid#113518PurposeExpresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processingDepositorInsertLbCas12a
UseTagsMBPExpressionBacterialMutationH759APromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lne_Cas13a_WT
Plasmid#91866PurposeBacterial expression for Cas13aDepositorInsertLneCas13a (LneC2c2) WT
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPP378
Plasmid#158797PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purificationDepositorInsertdCasPhi-1 (D371A)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CD3delta-CFP
Plasmid#128154PurposeExpression of CD3 deltaDepositorInsertCD3 Delta (Cd3d Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Ppr_Cas13a_WT
Plasmid#91872PurposeBacterial expression for Cas13aDepositorInsertPprCas13a (PprC2c2) WT
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-TIR1
Plasmid#83875PurposeGateway donor vector for TIR1DepositorInsertTIR1 (TIR1 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-MBP-TEV-Cas2
Plasmid#186275PurposeExpresses Thiomicrospira Cas2 in E. coliDepositorInsertThiomicropira Cas2
UseTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGP4G-TPLRD1-ccdB
Plasmid#83877PurposeDestination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assaysDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
L0-I39 PhiC31-Intein GG1-GG4
Plasmid#195905PurposeLevel 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkersDepositorInsertPhiC31-intein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only
