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Plasmid#147160PurposeInsect Expression of DmNot1-3xL-Amut-dsRNAresDepositorInsertDmNot1-3xL-Amut-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI
Plasmid#116955PurposeExpresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME nls EGFP-Cre (JDW 1329)
Plasmid#224515PurposeA Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusionDepositorInsertnls-EGFP-Cre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-del57-499-dsRNAres_J
Plasmid#146691PurposeInsect Expression of DmHPat-del57-499-dsRNAresDepositorInsertDmHPat-del57-499-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationone silent mutation A1758G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact-EGFP-3xHA (JDW 1322)
Plasmid#224494PurposeGateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)DepositorInsertLiefact-EGFP-3xHA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-EGFP-P2A-iCre (JDW 921)
Plasmid#224519PurposeA Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)DepositorInsertEGFP-P2A-iCre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)
Plasmid#224489PurposeGateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)DepositorInsertSun1-2xsfGFP-6xMYC-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME Actin-Vhh sfGFP (JDW 1215)
Plasmid#224495PurposeGateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)DepositorInsertActin-Vhh-sfGFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-E696K-EGFP
Plasmid#221554PurposeExpresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationChanged glutamate 696 to lysinePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
Plasmid#134868PurposeExpresses EGFP-tagged lysosomal markerDepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cFOS-FRB-VP16-P2A-EGFP
Plasmid#210510Purposeexpresses cFOS-FRB-VP16 and EGFP component in rat hippocampal neuron cellsDepositorInsertFRB-VP16-P2A-EGFP
UseTagsExpressionMammalianMutationPromotercFosAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-del698-725-dsRNAres_U
Plasmid#147703PurposeInsect Expression of DmNot3-del698-725-dsRNAresDepositorInsertDmNot3-del698-725-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-del723-745-dsRNAres_U
Plasmid#147705PurposeInsect Expression of DmNot3-del723-745-dsRNAresDepositorInsertDmNot3-del723-745-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690_R
Plasmid#147393PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690DepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690 (TNRC6C Nematode, Human)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-PAM26B-F1370A-dsRNAres_L
Plasmid#146838PurposeInsect Expression of DmGW182-PAM26B-F1370A-dsRNAresDepositorInsertDmGW182-PAM26B-F1370A-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-SD6B-F13170A-dsRNAres_L
Plasmid#146841PurposeInsect Expression of DmGW182-SD6B-F1370A-dsRNAresDepositorInsertDmGW182-SD6B-F1370A-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182_1-1199-delDUF-M2-dsRNAres_L
Plasmid#146821PurposeInsect Expression of DmGW182_1-1199-delDUF-M2-dsRNAresDepositorInsertDmGW182_1-1199-delDUF-M2-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delCterm-delM2-dsRNAres_L
Plasmid#146828PurposeInsect Expression of DmGW182-delCterm-delM2-dsRNAresDepositorInsertDmGW182-delCterm-delM2-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot3-del607-634-F673E_S
Plasmid#147503PurposeMammalian Expression of HsNot3-del607-634-F673EDepositorInsertHsNot3-del607-634-F673E (CNOT3 Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_862-1384_R
Plasmid#147392PurposeInsect Expression of CeAIN2_254-400-DmGW182_862-1384DepositorInsertCeAIN2_254-400-DmGW182_862-1384 (gw Fly, Nematode)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only