-
Plasmid#65511PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only
-
pFPL-Ch
Plasmid#61647PurposeGateway vector for generating cyan fluorescent protein fusions in fungiDepositorInsertenhanced cyan fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
LZRS PU.1 WT
Plasmid#34835DepositorInsertPU.1 (Spi1 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutationPromoterLTRAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF/myc/ER FLII12Pglu-700uDelta6
Plasmid#17867DepositorInsertFLII12Pglu-700uDelta6
UseTagsCitrineExpressionMammalianMutation700uDelta6, ECFP insert in mglBPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pXL-BACII-attPGAL4LWL
Plasmid#26260DepositorInsertGAL4 (GAL4 Budding Yeast)
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
ECYFP
Plasmid#111204Purposefluorescent fusion proteinDepositorInsertECFP-Xpress tag-GFP10C fusion
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXB1-pSG5
Plasmid#20981DepositorInsertHOXB1 (HOXB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
Twitch-1 pRSETB
Plasmid#48207PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-1
UseTags6xHIS, ECFP, and cpCit174ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
PU.1-W215G LZRS
Plasmid#44635DepositorInsertPU.1 W215G (Spi1 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutation215 mutation (W to G)PromoterAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPZP FLIPmal-25u
Plasmid#14476DepositorInsertFLIPmal
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationW230APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
PU.1-S148A LZRS
Plasmid#44633DepositorInsertPU.1 S148A (Spi1 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutation148 mutation (S to A)PromoterAvailable sinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPZP FLIPmal-control
Plasmid#14477DepositorInsertFLIPmal
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationW340APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFLIP32
Plasmid#65500PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP39
Plasmid#65507PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
PU.1-delta75-100 LZRS
Plasmid#44634DepositorInsertPU.1 Δ75-100 (Spi1 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutationdeletion of aa 75-100PromoterAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag Pu.1
Plasmid#66974PurposeExpression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of proteinDepositorInsertSpi1 (Spi1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Stx1a-12aa-mCer
Plasmid#53236PurposeFluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.DepositorInsertSyntaxin 1A (Stx1a Rat)
UseTags12aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Kras-Src FRET biosensor
Plasmid#78302PurposeFRET biosensor. To monitor Src kinase activity in mammalian cells by FRETDepositorInsertSrc biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW-4146NES(ROCK)
Plasmid#162485PurposeFor generating transgenic mice expressing ROCK biosensor based on FRET.DepositorInsertROCK biosensor based on FRET.
UseTagsYPet and ECFPExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only