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pET28aAviHisCD72aCTLD

Plasmid

#129065

Purpose

generation of biotinylated mouse CD72a CTLD protein using bacteria

Depositor

Takeshi Tsubata

Article

Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

Insert

CD72a (Cd72 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pET28aAviHisCD72cCTLD

Plasmid

#129066

Purpose

generation of biotinylated mouse CD72c CTLD protein using bacteria

Depositor

Takeshi Tsubata

Article

Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

Insert

CD72c (Cd72 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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bio-CD4d3+4-Kell blood group

Plasmid

#73089

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Kell blood group with bio-rat CD4d3+4

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Kell blood group (KEL Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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Basigin-isoform2-CD4d3+4-bio

Plasmid

#73129

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin isoform 2 (BSG Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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MRP1-CD4d3+4-bio

Plasmid

#73120

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding MRP1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

MRP1 (ABCC1 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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GlycophorinC-CD4d3+4-bio

Plasmid

#73096

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinC with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Glycophorin C (GYPC Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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Duffy-CD4d3+4-bio

Plasmid

#73122

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Duffy with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Duffy (ACKR1 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
bio-CD4d3+4-APMAP

Plasmid

#73091

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding APMAP with bio-rat CD4d3+4

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

APMAP (APMAP Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
JAM1-CD4d3+4-bio

Plasmid

#73110

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding JAM1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

JAM1 (F11R Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
bio-CD4d3+4-TMEM24

Plasmid

#73092

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding TMEM24 with bio-rat CD4d3+4

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

TMEM24 (C2CD2L Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Basigin-isoform1-CD4d3+4-bio

Plasmid

#73100

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin isoform 1 (BSG Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
NAR4-CD4d3+4-bio

Plasmid

#73118

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding NAR4 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

NAR4 (ART4 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Galectin3-CD4d3+4-bio

Plasmid

#73128

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Galectin3 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-bio-myc-NES-EGFP

Plasmid

#112712

Purpose

For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

EGFP

Use

Tags

HIV Rev nuclear localization signal (NES), biotin…

Expression

Mammalian

Mutation

Nucleotide sequence optimized for synthetic gene …

Promoter

CMV with Tet repressor binding sites

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-V5-APEX2 (in pLEX_307)

Plasmid

#214921

Purpose

constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

V5-APEX2

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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SYKA-c022

Plasmid

#175490

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Use

Tags

AviTag and His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG-hNFATc2/C - AVITEV

Plasmid

#74058

Purpose

retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc2, isoform C (NFATC2 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Mutation

silent mutation A1723C in the sequence of NM_1730…

Promoter

pMSCV-LTRs

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMIG-hH4-AVITEV

Plasmid

#74051

Purpose

retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Histone-H4 (H4C16 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG-hNFATc1/bC - AVITEV

Plasmid

#74059

Purpose

retroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc1, isoform beta-C (NFATC1 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Use

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge