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pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

Plasmid

#71214

Purpose

dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Use

Tags

dSpCas9-BFP-DNMT3B(E697A)

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-BFP HDRT Source (pTR 145)

Plasmid

#112014

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-BFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

Plasmid

#128166

Purpose

Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

mTagBFP2-T2A-sfGFP-iCre-ERT2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

Plasmid

#31478

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 K227M T2A BFP

Use

Lentiviral

Tags

HA and NLS

Expression

Mutation

F13Y, S111Y, K227M = Y2 nickase "reo" …

Promoter

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]

Plasmid

#133333

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All W to S in CTD of full-length TDP43

Promoter

Available since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

Plasmid

#102595

Purpose

Lentivector to express STING V155M delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V155M delta 354

Promoter

SFFV

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-BFP HDRT Source (pTR 176)

Plasmid

#112020

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-BFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

Plasmid

#178707

Purpose

AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

Dreadd-hM3Dq-HA-P2A-tBFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

Plasmid

#139230

Purpose

mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

Plasmid

#128755

Purpose

Expresses CYRANO gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

CYRANO sgRNA (OIP5-AS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

Plasmid

#126697

Purpose

donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA

Plasmid

#87574

Purpose

PV1 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP/BFP(LaG2) PAGER(FL)

Plasmid

#230022

Purpose

Expresses anti-GFP/BFP PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-LaG2-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

Plasmid

#163701

Purpose

Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Inserts

SOX2-3xHA-P2A-tagBFP (SOX2 Human)
rtTA
T2-sgRNA

Use

AAV and CRISPR

Tags

3x-HA and P2A-tagBFP

Expression

Mammalian

Mutation

Promoter

CAG and TRE3G

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myr-mTagBFP2-Flag (JDW 1183)

Plasmid

#224492

Purpose

Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

myr-mTagBFP2-Flag

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

Plasmid

#221082

Purpose

Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-msfGFP[r5M]_ACC-mEBFP2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

CMV, T7

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

Plasmid

#219688

Purpose

Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

Depositor

Jennifer Nemhauser

Insert

T2BP/p35S (BFP, mCherry, NeonGreen)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

Plasmid

#219687

Purpose

Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

Depositor

Jennifer Nemhauser

Insert

T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

Plasmid

#219686

Purpose

Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

Depositor

Jennifer Nemhauser

Insert

T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP(5X)

Plasmid

#218426

Purpose

Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

Depositor

Dieter Willbold

Article

Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27

Insert

GABARAP(G116A/5X)-mTagBFP2-GABARAP(5X) (GABARAP Human, Entacmaea quadricolor)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

5X: K2A, K13A, R15A, K20A, K23A mutations of both…

Promoter

CMV

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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