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Plasmid#116009Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVL42 - pLTP1>>
Plasmid#116008Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpLTP1:GR-LhG4:tLT1:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-Tol2
Plasmid#113383Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseTol2 reporterTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37deltaSEP
Plasmid#113501PurposeBacterial expression of GST-tagged p37 lacking the SEP domainDepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationdelta 140-206PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37delta2-81
Plasmid#113502PurposeBacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81DepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationdelta 2-81PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFL His-I3
Plasmid#113513PurposeExpression of His-I3 in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*HisExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M4
Plasmid#114036PurposeExpression of HEC1 M4 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M4 (NDC80 Human)
UseTagsExpressionMammalianMutationM4 (K146E, R153E, K156E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M2
Plasmid#114035PurposeExpression of HEC1 M2 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M2 (NDC80 Human)
UseTagsExpressionMammalianMutationM2 (K81E, N87E, K89E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
His Puc (aa 1-393)
Plasmid#52295PurposeBacterial expression of His tagged Drosophila Puc fragmentDepositorInsertPuc (puc Fly)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47A)
Plasmid#112722PurposeArg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R47A)
UseTags6His-TEVExpressionBacterialMutationR47APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47K)
Plasmid#112723PurposeArg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.DepositorInsert6His-TEV-TBP6.7(R47K)
UseTags6His-TEVExpressionBacterialMutationR47KPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48A)
Plasmid#112724PurposeGln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.DepositorInsert6His-TEV-TBP6.7(Q48A)
UseTags6His-TEVExpressionBacterialMutationQ48APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R52A)
Plasmid#112729Purpose52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.DepositorInsert6His-TEV-TBP6.7(R52A)
UseTags6His-TEVExpressionBacterialMutationR52APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(delta C-term)
Plasmid#112731PurposeThis mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.DepositorInsert6His-TEV-TBP6.7(delta C-term)
UseTags6His-TEVExpressionBacterialMutationdelta C-termPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK480] pLJC5 Flag-Depdc5(A)
Plasmid#109338PurposeLenti-viral expression of Flag-tagged Depdc5 mutant ADepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFLAGExpressionMutationDIYGD(185-189)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK481] pLJC5 Flag-Depdc5(B)
Plasmid#109339PurposeLenti-viral expression of Flag-tagged Depdc5 mutant BDepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFLAGExpressionMutationEQPLH(371-375)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK479] pLJC5 Flag-Depdc5(C)
Plasmid#109340PurposeLenti-viral expression of Flag-tagged Depdc5 mutant CDepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFLAGExpressionMutationFTPRI(419-423)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRA
Plasmid#108941PurposeMammalian expression of mRIN2 dRADepositorInsertRas and Rab interactor 2 (Rin2 Mouse)
UseTagsHAExpressionMammalianMutationDeletion of RA domain (aa 751-836)PromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRHVPS9
Plasmid#108942PurposeMammalian expression of mRIN2 dRHVPS9DepositorInsertRas and Rab interactor 2 (Rin2 Mouse)
UseTagsHAExpressionMammalianMutationDeletion of RHVPS9 domain (aa 455-738)PromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-dCEN-Incenp
Plasmid#108479Purposeexpression of vsv-dCEN-INCENPDepositorInsertdCEN-INCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationCEN box removedPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only