Skip to main content
Addgene
Showing: 10761 - 10780 of 34864 results

  13. Tol2-U6.3-sgRNA-empty-GFP

    Plasmid
    #221843
    Purpose
    empty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Article
    O’Sullivan et al bioRxiv 2024.03.04.583298
    Insert
    EGFP
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGC
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221843-plasmid-map-sequence-id-437471

    Enlarge

  14. Tol2-U6.3-sgRNA-DNM1L -GFP

    Plasmid
    #221845
    Purpose
    Tol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Article
    O’Sullivan et al bioRxiv 2024.03.04.583298
    Inserts
    EGFP
    DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGC and U6.3 chick
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221845-plasmid-map-sequence-id-437474

    Enlarge

  15. Tol2-U6.3-sgRNA-MFN1-GFP

    Plasmid
    #221846
    Purpose
    Tol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promote
    Depositor
    Stephen Terry
    Article
    O’Sullivan et al bioRxiv 2024.03.04.583298
    Inserts
    EGFP
    MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGC and U6.3 chick
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221846-plasmid-map-sequence-id-437473

    Enlarge

  17. P3-Lenti-dCas9-Tet1-GFP

    Plasmid
    #190729
    Purpose
    Lentiviral construct to express spdCas9-Tet1-GFP.
    Depositor
    Maja Jagodic
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    SV40 NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    UbC
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190729-plasmid-map-sequence-id-381312

    Enlarge

  18. pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)

    Plasmid
    #221330
    Purpose
    Dox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Insert
    FLAG-IRSp53 and HA-Rac1(G12V) (Baiap2, Rac1 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    G12V
    Promoter
    TRE3G
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221330-plasmid-map-sequence-id-439211

    Enlarge

  19. pMSCV-FKBP-MAVS(pLxIS) E433A

    Plasmid
    #221258
    Purpose
    Expression of MAVS pLxIS (393-441) mutant in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Insert
    MAVS pLxIS (393-441) (Mavs Mouse)
    Use
    Retroviral
    Tags
    FLAG-4xFKBP
    Expression
    Mutation
    E433A
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    221258-plasmid-map-sequence-id-439014

    Enlarge

Showing: 10761 - 10780 of 34864 results