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pExp-DsbC
Plasmid#112564PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-DsbC and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7TS-rich
Plasmid#99050PurposeThis plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.DepositorTypeEmpty backboneUseUnspecified; Xenopus oocyte systemTagsExpressionMutationPromoterpT7Available sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_hCOL4A4-3FLAG
Plasmid#198101Purposelentiviral expression of hCOL4A4 in mammalian cellsDepositorInsertCOL4A4 (COL4A4 Human)
UseLentiviralTags3FLAGExpressionMutationPromoterEF1aAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-jGCaMP8m-WPRE
Plasmid#176757PurposeAAV-mediated expression of GCaMP8m under the EF1a promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EnvARGCD
Plasmid#100800Purposeexpress fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domainDepositorInsertfusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 mRuby3-Rab18
Plasmid#166431PurposeRab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab18
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st
Plasmid#177232PurposeExpresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinaseDepositorInsertsgSik1_1st/sgSik2/sgSik3_1st
UseLentiviralTagsExpressionMutationPromoterbU6/mU6/hU6Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExp-MsyB
Plasmid#112570PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8His and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RNAseH1
Plasmid#65782Purposeexpresses FLAGHA-tagged RNAsH1 in mammalian cellsDepositorInsertRNaseH1 (RNASEH1 Zebrafish, Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
RAB5AA-c003
Plasmid#175475PurposeProtein expression in bacterial cells. Entire CDS, M1-N215.DepositorInsertRAB5A (RAB5A Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
bU6-sgMark2-mU6-sgMark3-hU6-sgMark4
Plasmid#177235PurposeExpresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinaseDepositorInsertsgMark2/sgMark3/sgMark4
UseLentiviralTagsExpressionMutationPromoterbU6/mU6/hU6Available sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2
Plasmid#177231PurposeExpresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinaseDepositorInsertsgNeo1/sgNT1/sgNeo2
UseLentiviralTagsExpressionMutationPromoterbU6/mU6/hU6Available sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sperm whale Mb 64Y68F in pVP80K
Plasmid#86956PurposeIncreases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).DepositorInsertMb 64Y68F
UseTagsExpressionBacterialMutation64Y68FPromoterT5Available sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pLIX403-EBFP2-ccdB-G418
Plasmid#158556PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-Kir2.1
Plasmid#114284PurposeTET-inducible expression of the Kir2.1 proteinDepositorInsertKir2.1 (KCNJ2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pB6-ROSA26-DTA-Capecchi-for
Plasmid#125743PurposeB6J ROSA26 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy numberDepositorTypeEmpty backboneUseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExp-TF
Plasmid#112575PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-TriggerFactor and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only
