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pcDNA5-puro-eGFP-HEC1-M2

Plasmid

#114035

Purpose

Expression of HEC1 M2 mutant tagged with GFP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

GFP tagged - HEC1 -M2 (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

M2 (K81E, N87E, K89E)

Promoter

CMV TetO

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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His Puc (aa 1-393)

Plasmid

#52295

Purpose

Bacterial expression of His tagged Drosophila Puc fragment

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

Puc (puc Fly)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only
pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK480] pLJC5 Flag-Depdc5(A)

Plasmid

#109338

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant A

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

DIYGD(185-189)GSGSG

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK481] pLJC5 Flag-Depdc5(B)

Plasmid

#109339

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant B

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

EQPLH(371-375)GSGSG

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK479] pLJC5 Flag-Depdc5(C)

Plasmid

#109340

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant C

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

FTPRI(419-423)GSGSG

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRA

Plasmid

#108941

Purpose

Mammalian expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRHVPS9

Plasmid

#108942

Purpose

Mammalian expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-dCEN-Incenp

Plasmid

#108479

Purpose

expression of vsv-dCEN-INCENP

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

dCEN-INCENP (INCENP Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

CEN box removed

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FNBP1-1/1

Plasmid

#91552

Purpose

Protein expression and purification of human SH3 domain construct FNBP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FNBP1-1/1 (FNBP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ABL2-1/1

Plasmid

#91533

Purpose

Protein expression and purification of human SH3 domain construct ABL2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ABL2-1/1 (ABL2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CACNB2-2/2

Plasmid

#91507

Purpose

Protein expression and purification of human SH3 domain construct CACNB2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB2-2/2 (CACNB2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CD2AP-3/3

Plasmid

#91508

Purpose

Protein expression and purification of human SH3 domain construct CD2AP-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CD2AP-3/3 (CD2AP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DLG5-1/1

Plasmid

#91522

Purpose

Protein expression and purification of human SH3 domain construct DLG5-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DLG5-1/1 (DLG5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CASKIN1-1/1

Plasmid

#91523

Purpose

Protein expression and purification of human SH3 domain construct CASKIN1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CASKIN1-1/1 (CASKIN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP26-1/1

Plasmid

#91484

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP26-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP26-1/1 (ARHGAP26 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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