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Cav-GA

Plasmid

#209880

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Use

Tags

CAVIN1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM253 - sgRNA mosTI hygroR

Plasmid

#159827

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI hygroR

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLPCX roGFP2-Prx2(C172A)

Plasmid

#158228

Purpose

Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

roGFP2-Prx2(C172A)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMYCO1_SpdCas9_pmcDA1

Plasmid

#204615

Purpose

CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-mCherry-GS linker-Zdk1

Plasmid

#125226

Purpose

Expresses mCherry-GS linker-Zdk1 in mammalian cells

Depositor

Alice Ting

Article

Kim et al Elife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

mCherry-GS linker-Zdk1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_213

Plasmid

#91112

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSEM270 - MCS mosTI hygroR target

Plasmid

#159826

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI hygroR target

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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HA-Cer-NCreIntN

Plasmid

#160505

Purpose

Expresses NCreIntN, tagged with HA, in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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yHS1

Plasmid

#159150

Purpose

Cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

yHS1

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4

Plasmid

#135460

Purpose

Mammalian expression of uFLARE TF component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape

Use

Tags

HA, flag,

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-NLGN2

Plasmid

#82545

Purpose

Overexpression vector for V5-NLGN2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

NLGN2 (Nlgn2 Rat)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Pinco-mNrp1

Plasmid

#21937

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Expression

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
epi-BE4max

Plasmid

#135975

Purpose

Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV syn CCR6-eLOV-TEVcs-tTA

Plasmid

#173116

Purpose

Expresses CCR6 receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-tTA

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-SLITRK3

Plasmid

#82539

Purpose

Lentiviral vector for expressing HRP-SLITRK3 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

SLITRK3 (SLITRK3 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-IntCCCre

Plasmid

#160506

Purpose

Expresses IntCCCre, tagged with FLAG, in mammalian cells in split intern-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

IntCCCre

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 tdAPX W41F

Plasmid

#40863

Purpose

Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

tandem dimer pea APX W41F

Use

Tags

Flag

Expression

Mammalian

Mutation

W41F mutation

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pAtCHYb1Δ129N

Plasmid

#53367

Purpose

Contains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

chyB1 (BETA-OHASE 1 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Deleted codons for the first 129 amino acids

Promoter

Trc

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA

Plasmid

#135462

Purpose

Neuronal expression of uFLARE TF component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA

Use

AAV

Tags

HA, flag, VP16

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pOGG010

Plasmid

#113984

Purpose

Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

RK2 (IncP)

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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