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FRT1-His-H2B-Cherry-2A (IG156)

Plasmid

#99622

Purpose

To clone gene of interest downstream of FRT1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

His-H2B-Cherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A24-VN

Plasmid

#135508

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*24:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*24:02 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC4A9

Plasmid

#132216

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A9 (SLC4A9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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attB-mCh-Dnmt3b1-Poly(A)-NeoR

Plasmid

#65553

Purpose

Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

mCherry

Expression

Mammalian

Mutation

isoform 1

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pAM4663

Plasmid

#85126

Purpose

NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.

Depositor

Susan Golden

Article

Chang et al Science. 2015 Jul 17;349(6245):324-8. do

Insert

PkaiB-luciferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAN19-3xFLAG-TIR1-NOSt

Plasmid

#108545

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDUAL-P41nmt1-Nup124-GFP-MTn

Plasmid

#154365

Purpose

Yeast expression of Nup124

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Nup124 (nup124 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

See depositor comments below

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Cherry-SRBC

Plasmid

#69492

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Gambin et al Elife. 2013 Jan 1;3:e01434. doi: 10.7554

Insert

SRBC (Cavin3 Mouse)

Use

Tags

Cherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only
Triple ORF FRT ifgMosaic Donor (IG135)

Plasmid

#99626

Purpose

To clone the 3 ORFs for FlpO dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

PGK-Neo

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT2-H2B-EGFP-V5-2A (IG157)

Plasmid

#99623

Purpose

To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A1-VN

Plasmid

#135502

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*01:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*01:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-135

Plasmid

#31067

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-135 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Dnmt3b1-Poly(A)-NeoR

Plasmid

#65551

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

isoform 1

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-NHL only-LIN-41

Plasmid

#52723

Purpose

retroviral expression of human LIN-41 / TRIM71 containing only the NHL repeats and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

NHL-only contains only an initiating methionine a…

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-7CtoA-LIN-41

Plasmid

#52726

Purpose

retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed cysteines 12, 15, 61, 66, 69, 91, and 94 …

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO-tet-puro-sh-ex16b

Plasmid

#35168

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
FRT3-Mb2-HA-mTfp1-2A (IG155)

Plasmid

#99621

Purpose

To clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2-HA-mTfp1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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FRT1-Mb2YFP-2A (IG153)

Plasmid

#99619

Purpose

To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2EYFP

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT2-Mb2Tomato-2A (IG154)

Plasmid

#99620

Purpose

To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2Tomato

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT3-HA-H2B-Cerulean-2A (IG158))

Plasmid

#99624

Purpose

To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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