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p7127 pHAGE-P-CMVt-N-HA-GAW-FTL

Plasmid

#100153

Purpose

Expresses FTL

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

FTL (FTL Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJP-HBB-nLuc

Plasmid

#175431

Purpose

Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nLUC plus 5' HBB leader

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1L

Plasmid

#33242

Depositor

Huda Zoghbi

Article

Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

Insert

ATXN1L (ATXN1L Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
sgLacZs

Plasmid

#171185

Purpose

Expresses sgRNA

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgLacZ1-hU6-sgLacZ2

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

U6, hSyn

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p199 Dync1i2.C (Ex1a)

Plasmid

#26449

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Tags

Expression

Mutation

N/A

Promoter

Available since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
pZac2.1 U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78603

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only
pSlick-Neo-RECK

Plasmid

#121986

Purpose

Tetracycline-inducible expression of untagged full-length KANK1

Depositor

GuangJun Zhang

Article

Kumari et al Oncotarget. 2018 May 4;9(34):23494-23504

Insert

RECK (RECK Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Tight TRE promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf40

Plasmid

#12728

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf40

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

Plasmid

#121976

Purpose

transfection studies in eukaryotic cells such as COS-7 cells

Depositor

Christophe Erneux

Article

De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

Insert

Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUDE735

Plasmid

#103024

Purpose

expression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST LIN28B

Plasmid

#43798

Depositor

Thomas Tuschl

Article

Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

Insert

LIN28B (LIN28B Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only

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Arch(D95H)-eGFP

Plasmid

#51081

Purpose

Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

Depositor

Adam Cohen

Article

Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi:

Insert

Archaerhodopsin-3 w/ D95H point mutation fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Histidine

Promoter

Ubiquitin

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51 GFP-PNRC1

Plasmid

#123295

Purpose

Mammalian expression of PNRC1 N-GFP

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 UBXN11

Plasmid

#169017

Purpose

Expresses GST-UBXN11 (human) in bacteria

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN11 (UBXN11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Gluc-EL222-his

Plasmid

#172097

Purpose

Expresses the Gluc-EL222 fusion protein in bacteria

Depositor

Avi Schroeder

Article

Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

Insert

Gaussia Luciferase - EL222

Use

Tags

Expression

Bacterial

Mutation

Gluc mutations - M43L, M110L

Promoter

T7

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 (N500)

Plasmid

#89897

Purpose

Expresses mouse TET2 N500 truncation in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

N-term 500 truncation of mouse TET2 (contains fir…

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-R399E

Plasmid

#122691

Purpose

Express His-tagged PKM2 (R399E) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Use

Tags

6His-tag

Expression

Bacterial

Mutation

R399E

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTrc99A-Rat FABP1

Plasmid

#13577

Depositor

Martin Scanlon

Article

Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

Insert

Rat liver fatty acid binding protein (Fabp1 Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only
HcRed-pcw107-V5

Plasmid

#64647

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only