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pCellFree_G03 YWHAB

Plasmid

#67063

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YWHAB (YWHAB Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pX330A_dCas9-1x5

Plasmid

#63599

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA2

Plasmid

#64984

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x3

Plasmid

#63597

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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MERS-CoV spike last4KO mutant

Plasmid

#231923

Purpose

For expression of MERS-CoV spike protein with glycan deletion at N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al npj Viruses. 2025 Jan; 3(4). doi: 10.103

Insert

spike last4KO (S MERS-CoV)

Use

Tags

Expression

Mammalian

Mutation

N1241Q, N1256Q, N1277Q, N1288Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCN38-kimA-yfp

Plasmid

#227635

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCN34e-ktrA-yfp

Plasmid

#227640

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCN34e-kimA-yfp

Plasmid

#227638

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCN38-ktrA-yfp

Plasmid

#227636

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0520 (pDEST, TRP1)

Plasmid

#210481

Purpose

pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Destination vector for gateway cloning

Tags

Expression

Mutation

Promoter

ADH1

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEPSA5Cas9syn

Plasmid

#206965

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Atg32 [200-341] 1B

Plasmid

#190431

Purpose

Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

Depositor

Michael Ragusa

Article

Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

Insert

Atg32

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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Atg11 [699-800] pHis2

Plasmid

#190264

Purpose

Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

Insert

ATG11

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Prepro SP

Plasmid

#153491

Purpose

S. cerevisiae Prepro Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Prepro SP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCuHA-SpAtg101(414)

Plasmid

#122081

Purpose

Expressing S. pombe HA-Atg101

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG101

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET30a-RAMA

Plasmid

#112226

Purpose

Expresses RAMA domain from murine MPND

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

MPND (Mpnd Mouse)

Use

Tags

Flag and HIs S-tag

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA5woH

Plasmid

#65019

Purpose

Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA2woH

Plasmid

#65018

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S69A

Plasmid

#51590

Purpose

expresses myc-tagged Rem2 S-A mutant

Depositor

Suzanne Paradis

Article

Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Serine 69 to Alanine; also RNAi resistant

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S334A

Plasmid

#51593

Purpose

expresses RNAiR myc-tagged Rem2 S-A mutant

Depositor

Suzanne Paradis

Article

Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Serine 334 to Alanine; also RNAi resistant

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only