Addgene

152,043 results

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pLucia

Plasmid

#135287

Purpose

GBPart - Lucia Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Lucia Luciferase

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTetR193-Wasabi

Plasmid

#86105

Purpose

mWasabi reporter gene gated by temperature-sensitive TetR I193N

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TetRts
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

I193N

Promoter

ptetA and ptetR

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFP95

Plasmid

#83550

Purpose

Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

PDA1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

PDA1

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only
Nrp2.3-AP-His

Plasmid

#71974

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.3 (Nrp2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pQE30-His-AlaRS

Plasmid

#124103

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Alanyl-tRNA ligase (alaS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHMGCS-Luc (aka: pES8)

Plasmid

#14947

Depositor

Axel Nohturfft

Article

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

HMG CoA synthase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_C4341S

Plasmid

#187156

Purpose

mammalian cell expression FLAG-HUWE C4341S

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

C4341S

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-SsAPOBEC3B

Plasmid

#198536

Purpose

Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-SsAPOBEC3B

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMax_GFP

Plasmid

#177825

Purpose

Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PKC alpha DN

Plasmid

#21235

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K368R

Promoter

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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