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BTK gRNA (BRDN0001146090)

Plasmid

#77073

Purpose

3rd generation lentiviral gRNA plasmid targeting human BTK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BTK (BTK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CMVTCRb_candidate6

Plasmid

#165002

Purpose

Expression of candidate TCR #6 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate6

Plasmid

#165001

Purpose

Expression of candidate TCR #6 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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FCIV1-GR S155A

Plasmid

#72702

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

S155A

Promoter

ubiquitin

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
CHEK2 gRNA (BRDN0001146385)

Plasmid

#76488

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHEK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CHEK2 (CHEK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CHEK2 gRNA (BRDN0001145828)

Plasmid

#76489

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHEK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CHEK2 (CHEK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K4 gRNA (BRDN0001145028)

Plasmid

#76650

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K4 (MAP2K4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K4 gRNA (BRDN0001145410)

Plasmid

#76651

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K4 (MAP2K4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDC6 B5.1 gRNA

Plasmid

#90596

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC6 (Guide Designation B5.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX301-RFP-GGA3 NGAT

Plasmid

#162031

Purpose

Expresses NGAT sequence of GGA3

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

GGA3 NGAT (GGA3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAWp40

Plasmid

#157980

Purpose

pFUGW-EFSp-BFP-{SbfI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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LYN gRNA (BRDN0001148376)

Plasmid

#77026

Purpose

3rd generation lentiviral gRNA plasmid targeting human LYN

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LYN (LYN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K8 gRNA (BRDN0001148662)

Plasmid

#75922

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K8

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K8 (MAP3K8 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K8 gRNA (BRDN0001146835)

Plasmid

#75923

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K8

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K8 (MAP3K8 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KMT5A H2.3 gRNA

Plasmid

#90727

Purpose

3rd generation lentiviral gRNA plasmid targeting human KMT5A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KMT5A (Guide Designation H2.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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ROR1 gRNA (BRDN0001145812)

Plasmid

#76045

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROR1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROR1 (ROR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_PSMD1_sgRNA_1

Plasmid

#155107

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting PSMD1 (core essential gene)

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

PSMD1_sgRNA_1 (PSMD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA13b_gb76_GF31-2-hom_lenti

Plasmid

#138310

Purpose

Lenti expression of AcrIIA13b

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA13b

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA15_gb79_Cand27_lenti

Plasmid

#138313

Purpose

Lenti expression of AcrIIA15

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14_gb77_Cand9_lenti

Plasmid

#138311

Purpose

Lenti expression of AcrIIA14

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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