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Plasmid#136833PurposeMammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tagDepositorInsertCLIP-Centrin2 (CETN2 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Centrobin
Plasmid#136830PurposeMammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tagDepositorInsertSNAP-Centrobin (CNTROB Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Cep72
Plasmid#136820PurposeMammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep72 (CEP72 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Cep57
Plasmid#136818PurposeMammalian expression of the centrosomal protein Cep57 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep57 (CEP57 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-Snrnp40
Plasmid#134255PurposeLentivector for Snrnp40 CRISPR knockoutDepositorInsertSnrp40 (Snrnp40 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationSnrnp40 gRNA “GATAACTATGCGACGTTGAA”PromoterU6Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-Flag-Snrnp40
Plasmid#134249PurposeLentivector encoding Flag-tagged Snrnp40DepositorInsertSnrp40 (Snrnp40 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pbIG-Xph20-Stg
Plasmid#133035PurposeExpresses Xph20-Stg in bacteriaDepositorInsertXph20-Stg
UseTagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph18-ETWV
Plasmid#133036PurposeExpresses Xph18-ETWV in bacteriaDepositorInsertXph18-ETWV
UseTagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph18-Stg
Plasmid#133037PurposeExpresses Xph18-Stg in bacteriaDepositorInsertXph18-Stg
UseTagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph15-ETWV
Plasmid#133038PurposeExpresses Xph15-ETWV in bacteriaDepositorInsertXph15-ETWV
UseTagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph0-ETWV
Plasmid#133039PurposeExpresses Xph0-ETWV in bacteriaDepositorInsertXph0-ETWV
UseTagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIG-TAT-Xph20-ETWV
Plasmid#133040PurposeExpresses TAT-Xph20-ETWV in bacteriaDepositorInsertXph20-ETWV
UseTagsHis8, TAT, and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONRG_P4-P1R:TBA2p
Plasmid#128429PurposeGateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.DepositorInsertTESTA ABUNDANT 2 (AT1G62060 Mustard Weed)
UseGateway promoter entry cloneTagsExpressionMutationPromoterTESTA ABUNDANT 2Available sinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTAMAHISTEV_PfeAR480A
Plasmid#128944Purposeexpresses R480A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAQ482A
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAG324V
Plasmid#128947Purposeexpresses G324V mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des1
Plasmid#110212PurposeYeast Surface Display of G6des1DepositorInsertG6des1
UseTagscmyc tagExpressionYeastMutationL A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…PromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Designed HEL-binding scFv D44.1des
Plasmid#111719PurposeYeast Surface Display of D44.1desDepositorInsertD44.1 des
UseTagscmyc tagExpressionYeastMutationL Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…PromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]
Plasmid#124068PurposeCRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)DepositorInserteb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40
UseCRISPR and Cre/LoxTagsExpressionInsectMutationPromoterbhsp68Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only