-
Plasmid#72573PurposeExpresses myc-PICK1 shRNA resistant cDNADepositorInsertPICK1 (Pick1 Rat)
UseTagsMycExpressionMammalianMutation5 silent mutations around shRNA recognition seque…PromoterCMVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
LZF80: Cre-on CAG-somCheRiff-CFP (DIO)
Plasmid#104115PurposesynOptopatch constructDepositorInsertsomCheRiff-CFP
UseLentiviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-MRPL50-VC155
Plasmid#182373PurposemVenus-based mito-riboBiFC vector (Negative control)DepositorInsertMrpl50 (Mrpl50 Mouse)
UseTagsVC155ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-MRPS6-VN172
Plasmid#182374PurposemVenus-based mito-riboBiFC vectorDepositorInsertMrps6 (Mrps6 Mouse)
UseTagsVN172ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15bLCFPT7
Plasmid#53545PurposeActivation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistantDepositorInsertT7 RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDCC5
Plasmid#59984PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassetteDepositorInsertCas9-D10A
UseCRISPRTagsHAExpressionInsectMutationD10APromoterhsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTK298
Plasmid#59567PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4DepositorInsertSGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenticrispr-wt-ltr-puro
Plasmid#173428PurposeExpresses Cas9 and guide RNA, contains intact LTRDepositorInsertS. pyogenes sgRNA cassette
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomRuby-SpHis5
Plasmid#44843PurposeYeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selectionDepositorInsertyomRuby
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PP255
Plasmid#78934PurposePromoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJB01
Plasmid#111957PurposeExpresses His10-RFP-Halo; construct generated for pull-down and localization experimentsDepositorInsertRFP
UseTagsHaloTagExpressionBacterialMutationPromoterT7Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEos2-SpHis5
Plasmid#44849PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with SpHis5 selectionDepositorInsertyomEos2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro SARS-CoV2 N P2A eGFP-NLS
Plasmid#154399PurposePiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFPDepositorInsertSARS-CoV2 N Protein (N SARS-CoV2)
UsePiggybacTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_sfCherry2(11)_Keratin
Plasmid#82604PurposeExpresses sfCherry2(11) tagged keratin in mammalian cellsDepositorInsertsfCherry2(11)_Keratin
UseTagsExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+PromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
untargeted Aurora B FRET sensor Kif2 substrate
Plasmid#45215DepositorInsertuntargeted Aurora B FRET sensor
UseTagsCyPET, YPETExpressionMammalianMutationPromotert7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA4-lenti
Plasmid#115663PurposeExpresses AcrVA4 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA4
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEF-1a promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
nonphosphorylatable Mis12-targeted Aurora B FRET sensor
Plasmid#45229DepositorInsertnon phosphorylatable Mis12-targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationT-A mutation in substrate sequencePromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pK3F-GAL1
Plasmid#85772PurposeN-ICE plasmid pK3F-GAL1DepositorInsertloxP-KanMX-loxP-GAL1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTYF-EF1a-DLDH-IRES-EGFP
Plasmid#178310PurposeExpress the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cellsDepositorInsertD-lactate dehydrogenase
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSTEP
Plasmid#72334PurposeTissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[Golic+] RRS-EGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only