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pJJF152_Phsp_Cas9

Plasmid

#163862

Purpose

heat shock-induced expression of Cas9 in C. elegans

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

Cas9, C. elegans codon optimized with intron and SV40_NLS

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Phsp-16.48

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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sg1

Plasmid

#113966

Purpose

Single short guide RNA targeting GTATAGCATACATTATACG

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg1

Use

Tags

Expression

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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C-13-BE

Plasmid

#135041

Purpose

C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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C-7-BE

Plasmid

#135035

Purpose

C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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C-6-BE

Plasmid

#135034

Purpose

C-T base editing using LjDA1L2_2 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjDA1L2_2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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C-11-BE

Plasmid

#135039

Purpose

C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L2_2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-HypaCAS9-pA

Plasmid

#131467

Purpose

Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

Insert

Codon optimized HypaCas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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PV521

Plasmid

#132346

Purpose

Cas9 R promoter targeting sgRNA1 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

Cas9 R promoter targeting sgRNA1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV523

Plasmid

#132347

Purpose

Cas9 R promoter targeting sgRNA2 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

Cas9 R promoter targeting sgRNA2

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV528

Plasmid

#132348

Purpose

Cas9 R promoter targeting sgRNA3 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

Cas9 R promoter targeting sgRNA3

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV564

Plasmid

#132349

Purpose

SthCascade R promoter targeting crRNA1 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

SthCascade R promoter targeting crRNA1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV565

Plasmid

#132350

Purpose

SthCascade R promoter targeting crRNA2 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

SthCascade R promoter targeting crRNA2

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV569

Plasmid

#132351

Purpose

SthCascade R promoter targeting crRNA3 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

SthCascade R promoter targeting crRNA3

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV RhoB YFP MS2

Plasmid

#131777

Purpose

RhoB upstream msfYFP 24xMS2 RhoB downstream for CRISPR tagging

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

msfYFP - 24x MS2 loops

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV Btg2 YFP MS2

Plasmid

#131776

Purpose

Btg2 upstream msfYFP 24xMS2 Btg2 downstream for CRISPR tagging

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

msfYFP - 24x MS2 loops

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLR5-U6-sgRNA-EF

Plasmid

#82635

Purpose

piggyBac vector for expressing modified sgRNA in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Type

Empty backbone

Use

CRISPR; Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFH50

Plasmid

#128212

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK007

Plasmid

#128220

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH72

Plasmid

#128221

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH73

Plasmid

#128222

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge