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Showing: 10661 - 10680 of 11049 results

  7. pPICZ:K2P2.1(mTREK-1)Cryst S112C

    Plasmid
    #224780
    Purpose
    Pichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoter
    Depositor
    Dan Minor
    Article
    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1
    Insert
    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    Use
    Tags
    Expression
    Yeast
    Mutation
    K84R / Q85E / T86K / I88L / A89R / Q90A / A92P / …
    Promoter
    AOX1 promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224780-plasmid-map-sequence-id-445194

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  9. pLX_EFS-Cre_ppt-del

    Plasmid
    #162073
    Purpose
    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Insert
    Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EFS (part of insert)
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162073-plasmid-map-sequence-id-325292

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  12. pINSPECT:IL2_SigP:NLuc

    Plasmid
    #197266
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    Interleukin-2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197266-plasmid-map-sequence-id-389716

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  13. pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

    Plasmid
    #175549
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.
    Depositor
    Elzo de Wit
    Article
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Insert
    WAPL (Wapl Mouse)
    Use
    Mouse Targeting
    Tags
    AID[71-114]-eGFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175549-plasmid-map-sequence-id-345088

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  14. p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)

    Plasmid
    #52216
    Purpose
    mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and LCLL mutation
    Depositor
    Eric Huang
    Article
    Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2
    Insert
    HIF1alpha (401delta603) LCLL (HIF1A Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    aa 401-603 deleted, removes the oxygen-dependent …
    Promoter
    CMV
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pcDNA3.1-KozATG-dL5-2XG4S-actin

    Plasmid
    #73262
    Purpose
    Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.
    Insert
    KozATG-dL5-2XG4S-actin (ACTB Synthetic, Human)
    Use
    Tags
    The FAP and actin are fused with 2 copies of a G4…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73262-plasmid-map-sequence-id-137068

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  17. pBabe-kappa-HA-dL5-myc-ADRB2

    Plasmid
    #101253
    Purpose
    Expresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)
    Depositor
    Marcel Bruchez
    Article
    Perkins et al Biochemistry. 2018 Feb 6;57(5):861-871.
    Insert
    kappa-HA-dL5-myc-ADRB2 (ADRB2 Budding Yeast, Human)
    Use
    Retroviral
    Tags
    FAP-dL5 and HA epitope
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV LTR
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    101253-plasmid-map-sequence-id-235286

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  18. pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2

    Plasmid
    #169833
    Purpose
    Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 and F1366 - P1376 in allosteric domain
    Depositor
    Richard Possemato
    Article
    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422
    Insert
    CAD (CAD Human)
    Use
    Retroviral
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Deletions of amino acids R1398 - S1407 and F1366 …
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    169833-plasmid-map-sequence-id-339176

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  19. pMXS-IRES-BLAST flag-CAD delta Allosteric Domain

    Plasmid
    #169835
    Purpose
    Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker
    Depositor
    Richard Possemato
    Article
    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422
    Insert
    CAD (CAD Human)
    Use
    Retroviral
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Substitution of amino acids F1308 - C1455 with a …
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    169835-plasmid-map-sequence-id-333093

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Showing: 10661 - 10680 of 11049 results