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Plasmid#64993PurposeMammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)DepositorInsertOrp1 (HYR1 Budding Yeast)
UseLentiviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
mycBioID2-pBABE-puro
Plasmid#80900PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); a small MCS is present downstream of myc tag and BioID2 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagshumanized A. aeolicus BioID2 with R40G and mycExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-GCaMP3
Plasmid#64854PurposeEndoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-GCaMP3
UseTagsN-terminal 27 amino acids from CYP450ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PM-B
Plasmid#209876PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddGFP B
UseTagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-NTEVp(WT)
Plasmid#137831PurposeCytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV proteaseDepositorInsertFKBP-NTEVp(WT)
UseTags3x FLAG and NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB-CTEVp(WT)
Plasmid#137832PurposeCytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV proteaseDepositorInsertFRB-CTEVp(WT)
UseTags3x FLAG and NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
LD-B
Plasmid#209861PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddGFP B
UseTagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyso-GA
Plasmid#209874PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP A
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM134
Plasmid#127215PurposeBeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDSDepositorInsertLuc, IPT, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lyso-RA
Plasmid#209875PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddRFP A
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-V5_pRS415
Plasmid#107168Purposeexpresses V5-tagged miniTurbo in the yeast cytosolDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionYeastMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-TurboID-V5_pCDNA3
Plasmid#107173Purposeexpresses V5-tagged TurboID on the mammalian ER membraneDepositorInsertTurboID (BirA mutant)
UseTagsExpressionMammalianMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
UseTagsExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM135
Plasmid#127216PurposeBeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDSDepositorInsertLuc, WUS2, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
LOV-Turbo-His6_pET21a
Plasmid#199668Purposeexpresses LOV-Turbo in bacteriaDepositorInsertLOV-Turbo
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPa-NRX
Plasmid#73143PurposeLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.DepositorInsertsHRPa-NRX
UseTags2x HA tag and FlagExpressionMammalianMutationPromoterCAGAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
PM-GA
Plasmid#209877PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddGFP A
UseTagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-TurboID-V5_pLX304
Plasmid#107175Purposeexpresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vectorDepositorInsertTurboID (BirA mutant)
UseLentiviralTagsExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209724PurposeExpresses cytosolic low affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95A.A119L.HaloTag
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyso-B
Plasmid#209873PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP B
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only