-
Plasmid#121007PurposeMoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).DepositorInsertmScarlet-I codon optimized with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
6NEY
Plasmid#127295PurposeBacterial expression for structure determination. May not contain entire coding region of geneDepositorInsertPP-BRD3
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
6MF9
Plasmid#127290PurposeBacterial expression for structure determination. May not contain entire coding region of geneDepositorInsertPP-BRD12
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his-24::mOrange
Plasmid#124346PurposeNuclear Localized Pan-Neuronal mOrangeDepositorInsertrab-3, mOrange (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1-lim-4P::his-24::Cerulean
Plasmid#124336PurposeMulticolor Worms for Neuronal IDDepositorInsertlim-4, Cerulean (lim-4 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 2
Plasmid#109221PurposeNon binary level 1 position 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9CR5
Plasmid#120227PurposepCas9CR4 with stop codon to prevent translation of ssrA degradation tagDepositorInsertSpCas9
UseSynthetic BiologyTagsExpressionMutationStop codon inserted before ssrA degradation tagPromoterpTetAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJR288
Plasmid#78550PurposeFor expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 6
Plasmid#109225PurposeNon binary level 1 position 6 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
MoClo adapted pUC57-Amp Level 1 position 4
Plasmid#109223PurposeNon binary level 1 position 4 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 1
Plasmid#109220PurposeNon binary level 1 position 1 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 5
Plasmid#109224PurposeNon binary level 1 position 5 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 3
Plasmid#109222PurposeNon binary level 1 position 3 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL9-YFPc-1
Plasmid#102400Purposesplit YFP. Plant expression of CBL9-YFPc-1DepositorInsertCBL9 (CBL9 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL8-YFPc-1
Plasmid#102399Purposesplit YFP. Plant expression of CBL8-YFPc-1DepositorInsertCBL8 (CBL8 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL5-YFPc-1
Plasmid#102397Purposesplit YFP. Plant expression of CBL5-YFPc-1DepositorInsertCBL5 (CBL5 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-Slah3-YFPn-1
Plasmid#102385Purposesplit YFP. Plant expression of Slah3-YFPn-1DepositorInsertSLAH3 (SLAH3 Mustard Weed)
UseTagsYFPnExpressionPlantMutationPromoter35SAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gapAP1
Plasmid#87146PurposegapAP1 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgapAP1 guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pREP81xV5_ccdB
Plasmid#79019PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selctionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only