Addgene

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pCW57.1 DOX off blast NFS1

Plasmid

#160801

Purpose

Express Dox repressible NFS1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

NFS1 (NFS1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-SwitchON-sgCh2-2

Plasmid

#199637

Purpose

Tamoxifen-inducible expression of sgRNA control targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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sgCTRL-hygro

Plasmid

#199639

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC51A

Plasmid

#132240

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC51A (SLC51A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC51B

Plasmid

#132157

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC51B (SLC51B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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XLone-Puro Cas13d P2A eGFP-NLS

Plasmid

#155182

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

CasRx

Use

Unspecified; Piggybac transposon

Tags

NLS and P2A eGFP NLS

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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EPHB4-2A-EGFP

Plasmid

#139968

Purpose

Donor vector to knockin EGFP (separated by 2A) into EPHB4 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

Human EPHB4 knockin homology arms (EPHB4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro shPOLE1_1

Plasmid

#160810

Purpose

Express Dox repressible shPOLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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tdEos-Mito-7

Plasmid

#57644

Purpose

Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Mito (COX8A Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAGE2.0

Plasmid

#165984

Purpose

Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.

Depositor

Bogumil Karas

Article

Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

Inserts

RK2/RP4 origin of transfer
repA2B2C2
nourseothricin N-acetyl transferase
tetracycline resistance

Use

Synthetic Biology; Bacterial and yeast cloning; c…

Tags

Expression

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mature ISG15 C-term. domain (79-157)

Plasmid

#110764

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC38A10

Plasmid

#132083

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A10 (SLC38A10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC7A14

Plasmid

#132173

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A14 (SLC7A14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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HNF4A_P2-285

Plasmid

#31060

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR221_SVOP

Plasmid

#132305

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SVOP (SVOP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SPNS2

Plasmid

#132307

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SPNS2 (SPNS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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attB-GFP-Dnmt3b6-Poly(A)

Plasmid

#65532

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

isoform 6

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pLV V5-TbID-emerin

Plasmid

#175099

Purpose

Lentiviral expression of V5-TbID-tagged mouse Emd

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Emerin (Emd Mouse)

Use

Lentiviral

Tags

V5-TbID

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ269A2

Plasmid

#164714

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Med26

Plasmid

#49250

Purpose

Mammalian expression of flag-tagged Med26

Depositor

Ronald Conaway, Joan Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only