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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

Plasmid

#55216

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-pDEST_TMEM192_BirA_Flag

Plasmid

#227958

Purpose

BioID experiment

Depositor

Alessandro Ori

Insert

TMEM192 (TMEM192 Human)

Use

Tags

BirA-FLAG

Expression

Mammalian

Mutation

R118G in BirA

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pOGS539_GLuc-ns

Plasmid

#226719

Purpose

Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Gaussia Luciferase

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

pTEF1

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-NG-CBE C

Plasmid

#227422

Purpose

expresses the C-terminal of AAV-NG-CBE

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AAV-NG-CBE C-terminal

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p36.pA.NGFR.mCMV.hPGK.mGLuc(flag).WPRE[sequenced]

Plasmid

#226731

Purpose

backbone vector

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-5102HRasCT(Picchu)

Plasmid

#209917

Purpose

A FRET biosensor for tyrosine kinase activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

5102HRasCT

Use

Tags

YPet and nTurquoise-GL

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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p34.pA.NGFR.mCMV.hPGK.mSrtA(Ge)TM.WPRE[sequenced]

Plasmid

#226759

Purpose

sortase A (GE) - TM in p36

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

sortase A (srtA )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUASTattB-dALFANb(5mut)-mGreenLantern

Plasmid

#219661

Purpose

Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-Lrrc59

Plasmid

#226565

Purpose

Encodes Lrrc59 protein laebeled with mCherry

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-Lrrc59

Plasmid

#226564

Purpose

Encodes Lrrc59 protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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